环磷酰胺诱发的染色体损伤与P53基因表达的关系  

RELATIONSHIP BETWEEN CHROMOSOME DAMAGE INDUCED BY CYCLOPHOSPHAMIDE AND EXPRESSION OF MUTATED P53 GENE

作　　者：王明艳[1] 许冬青[1] 徐力[2] 张旭[1] 赵凤鸣[1] 姜海英[1] 

机构地区：[1]南京中医药大学基础医学院生物教研室,江苏南京210029 [2]南京中医药大学学报编辑部,江苏南京210029

出　　处：《癌变．畸变．突变》2003年第4期228-230,共3页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：江苏省教育厅自然科学基金资助项目(KJB360006)

摘　　要：目的 :探讨环磷酰胺(cyclophosphamide,CP)对染色体损伤与P53基因表达间的关系。 方法 :以正常人外周血淋巴细胞为材料 ,用流式细胞仪检测环磷酰胺对突变型P53基因表达的影响 ,同时以微核(MN)及姐妹染色单体互换(SCE)为指标 ,进行致突变研究。 结果 :环磷酰胺用药组突变型P53基因表达率为30.81 % ,微核率为8‰ ,姐妹染色单体互换率为9.21±1.08 ,与对照组比较均有显著差异。 结论Purpose: To study the relation between chromosome damage induced by cyclophosphamide(CP) and the expression of mutated P53 gene. Methods: The expression of mutated P53 gene in human lymphocytes was investigated using flow cytometry.And the micronucleus(MN) and sister chromatid exchange (SCE) tests were studied in vitro. Results: The expression ofmutated P53 gene in human lymphocytes treated with CP was 30.81 %,(P < 0.01)compared with the control .The frequencies of SCE and MN in human lymphocytes exposed with CP were 9.21±1.08 and 8 ‰ respectively(P < 0.01),compared with the control. Conclusion: The mutagenic effect of CP may be related to the expression of mutated P53 gene .

关 键 词：环磷酰胺 致突变 突变型P53基因 微核 姐妹染色单体互换 

分 类 号：R285.5[医药卫生—中药学]