云南战区部队首次检出O_(157)∶H_7大肠杆菌  

Detection of O_(157):H_7 E. coli for the first time in troops in Yunnan Province

作　　者：李刚山[1] 范泉水[1] 邱薇[1] 郭兆彪[2] 唐火明[3] 龙沛然[1] 孙阳[1] 尹惠琼[1] 杨美峰1 

机构地区：[1]成都军区联勤部军事医学研究所 [2]军事医学科学院微生物流行病研究所 [3]中国人民解放军77223部队卫生队

出　　处：《中国卫生检验杂志》2003年第5期602-604,共3页Chinese Journal of Health Laboratory Technology

摘　　要：〔目的〕了解EHECO157∶H7在云南战区部队腹泻患者和战区猪、牛和污水中的存在情况。〔方法〕在SMAC琼脂平板上分离 ,快速MUG试验初筛 ,用VITEK3 2 全自动微生物生化分析仪鉴定 ,复合PCR扩增法检测毒素基因。〔结果〕从人、猪、牛中分别检出EHECO157∶H7和EHECO157:H-,其中O157:H7检出三个毒力基因。〔结论〕云南战区至少存在两种不同类型的EHECO157,要警惕和预防此菌的感染。〔objective〕 To detect the EHEC O 157∶H 7 in the of stool army diarrhea patients, pigs and cows and in polluted water in yunnan war zone.〔Methods〕 Samples Were separated by SMAC agar plate then fast MUG test for the initial screen and appraisement by VlTEK32 auto-microbes' biochemistry analysis apparatus. Muti-PCR to detect the genotype of toxin.〔Results〕 EHEC O 157∶H 7(three strains)and EHEC O 157:H -(three strains)were detected in the stool of patients, pigs and cows. Three toxin genotypes of O 157∶H 7 were detected 〔Conclusions〕there are at least two types of EHEC O 157 in Yunnan war zone. we should on guard against and prevent theinfection of this bacterium.

关 键 词：云南战区 部队卫生 O157:H7大肠杆菌 腹泻 PCR 毒力基因 毒力试验 

分 类 号：R824.2[医药卫生—临床医学] R378.21