血清中假尿苷含量的高效液相色谱分析方法研究  

The study on concentration of pseudouridine in the human serum by HPLC analytic method

作　　者：李杰[1] 王景萍[1] 周丽莉[1] 王玲 孔永霞[1] 丁亚[1] 

机构地区：[1]河南省肿瘤研究所,河南郑州450003

出　　处：《河南医学研究》2003年第3期226-227,共2页Henan Medical Research

摘　　要：目的 :建立一种快速、准确的血清中假尿嘧啶核苷高效液相色谱检测方法。方法 :采用Spherigel C18色谱柱 ;流动相为含 0 5 %乙腈的 0 0 2mol/LKH2 PO4缓冲液 ;流速 0 95ml/min ;检测波长 2 5 4nm。结果 :线性范围 1～ 16mg/L ;相关系数r =0 .9986;平均回收率 98% ;最低检测浓度 0 3mg/L。结论 :本法具有样品处理简单、稳定性高等特点 。Objective: To establish a rapid and precise method for the determination of concentration of pseudouridine in the human serum.Methods: The concentration of pseudouridine was assayed on a Spherigel-C 18 column with a mobile phase consisting of acetonitrile:0.02 mol/L KH 2PO 4(5∶1000) at a flow rate of 0.95 ml/min and detected at 254 nm.Results: The calibration curve was linear over the range of 1～16 mg/L(r=0.9986).The average recovery of hypoxanthine was 98%.The limit of quantification was 0.3 mg/L.Conclusion: This method possessed the features of simple sample treatment and high stability.Clinical application demonstrated that this method had a better practicality and was popularized easily.

关 键 词：血清学检测 高效液相色谱 假尿嘧啶核苷 样品处理 

分 类 号：R446.11[医药卫生—诊断学]