腐蹄病A型节瘤拟杆菌纤毛蛋白基因的克隆与序列分析  

Cloning and Sequencing of Pili Gene of D.nodosus Serotype A in Footrot

作　　者：张洪涛[1] 陈立志[1] 苗立光[1] 刘晓颖[1] 王克坚[2] 

机构地区：[1]中国农业科学院特产研究所,吉林吉林132109 [2]厦门大学,福建厦门361005

出　　处：《特产研究》2003年第3期8-10,共3页Special Wild Economic Animal and Plant Research

基　　金：国家科技部攻关计划"奶牛主要疫病防治关键技术研究与产业化开发"项目(2002BA518A04)

摘　　要：以腐蹄病A型节瘤拟杆菌染色体DNA为模板,根据设计的上、下游引物进行PCR反应,扩增出了大小约0.78kb的基因片段,用T_4DNA连接酶将PCR产物与pGEM-T-Easy载体连接,构建了重组质粒。并以EcoRV和SalI双酶切重组质粒鉴定目的基因的插入方向。经序列测定,克隆的目的基因片段为Pili基因。The pili gene that dominates the main protective antigen was amplified and cloned from D. nodosus serotype A by PCR. A recombinant plasmid,designated TE- Pili,was constructed by inserting the Pili gene intoT - Easy which was digested with EcoRV and Sal I. The result of sequencing showed that the fragment was exactly Pili gene of D. nodosus serotype A.

关 键 词：腐蹄病 A型节瘤拟杆菌 纤毛蛋白基因 基因克隆 序列分析 反刍动物 

分 类 号：S858.23[农业科学—临床兽医学] S852.61[农业科学—兽医学]