dsRNA引发的基因沉默  

dsRNA-triggered gene-silencing

作　　者：周玲艳[1] 秦华明[2] 谢振文[1] 

机构地区：[1]仲恺农业技术学院农学系,广东广州510225 [2]暨南大学环境工程系,广东广州510641

出　　处：《仲恺农业技术学院学报》2003年第3期59-66,共8页Journal of Zhongkai Agrotechnical College

摘　　要：双链RNA(double strandedRNA,dsRNA)导入细胞后引起细胞内特定的信使核糖核酸(mes sengerRNA,mRNAs)降解,从而导致基因沉默,并使生物体产生相应的功能缺陷型,这种由dsRNA介导的基因沉默称为RNA干涉(RNAi),RNAi普遍存在于植物、动物、真菌中,它的发现改变了人们对细胞如何保护其基因组的理解,并发展了研究基因功能的新战略.对RNAi的发现历史、作用特点、作用机制以及应用前景进行了简述.The introduction of double strand RNA(dsRNA) into cell can target specific mRNAs for degradation,thereby silencing their expression, and emerging the lossoffunction phenotype, the phenomenon of genesilencing mediated by dsRNA is called RNA interference(RNAi) which are found in plants, animals and fungi. The discovery of RNAi has changed our understanding of how cells guard their genomes, and led to the development of new strategies for studying gene function. The discovery, characteridstic, machanism and application of RNAi were discussed in this paper.

关 键 词：DSRNA 基因沉默 生物体 生长发育 防御 转座子 病毒 RNA干涉 核糖核酸 

分 类 号：Q522.2[生物学—生物化学] Q78