五倍子在体外生物膜模型中对口腔常驻菌形成的致龋生物膜的影响  

Evaluation of in vitro Cario-static Effect of Galla chinensis with Biofilm Model

作　　者：李继遥[1] 黄正蔚[2] 周学东[3] 刘天佳[3] 

机构地区：[1]四川大学 [2]上海第二医科大学附属第九人民医院口腔研究所 [3]教育部口腔生物医学工程教育部重点实验室,610041

出　　处：《华西口腔医学杂志》2003年第5期389-391,共3页West China Journal of Stomatology

基　　金：国家自然科学基金 (编号 30 1 71 1 75);四川省教育厅国际合作项目基金 (编号 41 30 2 0 )资助项目

摘　　要：目的　研究五倍子对口腔常驻菌形成的人工致龋生物膜的影响 ,探讨其防龋应用前景。方法　在体外模型中的羟基磷灰石片上定植 4种口腔常驻菌 ,以形成实验性生物膜。实验组定时给予质量浓度 4g L的五倍子提取物的蔗糖溶液处理生物膜 ,观察其pH的动态变化 ,生物膜表面微结构与微生物组成的改变。以同质量浓度的蔗糖溶液与蒸馏水分别作为阳性与阴性对照。结果　①实验组培养室内pH值呈先下降 ,再逐步恢复正常的波浪形变化 ;②五倍子的加入能抑制变形链球菌对羟基磷灰石片的粘附 ;③五倍子的加入可使生物膜表面胞外基质的量减少。Objective\ To evaluate the in vitro cario_static effect of Galla chinensis with biofilm model. Methods\ A four_organism bacterial consortium was cultured in a biofilm model on hydroxyapatite (HA) discs in a continuous culture system and exposed to repeated solution pulsing. There were three groups with different solution pulsed in the model: negative control group was pulsed with distilled water, positive control group was pulsed with 100 mmol/L sucrose solution and experimental group was pulsed with 100 mmol/L sucrose solution containing 4 0 g/L Galla chinensis. During the experiment, the dynamic changes of pH were recorded. After 6 pulses, surface structure of the biofilm was observed with a scanning electron microscope and the population on the biofilm was enumerated.Results\ Galla chinensis significantly inhibited the adherence of Actinomyces naelundii to HA disc compared with the control group and facilitated the removal of acid products. It was also found that the extra_cellular polysaccharide was reduced with the pulsing of Galla chinensis.Conclusion\ Galla chinensis in the biofilm model can partially reduce the cario_genic response of sucrose solution.

关 键 词：五倍子 口腔常驻菌形成 龋病 生物膜 体外模型 

分 类 号：R285.5[医药卫生—中药学]