用一次性滤除白细胞输血器回收PBMC制备DC的实验研究  

Obtained DC from PBMC recovered from one time used no leukocytes transfusion apparatus

作　　者：郭建巍[1] 秦力维[2] 吕同德[1] 王燕宁[3] 李秀萍[3] 

机构地区：[1]兰州军区兰州总医院临床实验科,兰州730050 [2]兰州军区兰州总医院眼科,兰州730050 [3]兰州军区兰州总医院输血科,兰州730050

出　　处：《中国免疫学杂志》2003年第10期707-708,712,共3页Chinese Journal of Immunology

摘　　要：目的 :从一次性滤出白细胞输血器回收PBMC ,诱导DC。方法 :用冲洗法从一次性滤出白细胞输血器回收PB MC ,使用GM CSF和IL 4两种细胞因子 ,诱导 7天获取DC。结果 :平均从每个白细胞输血器中回收到 5 1× 10 7PBMC ,诱导出4 7× 10 6 CD83+ DC ,诱导出的DC具有明显的树突状突起 ,纯度为 96 9%。DC高表达CD86 (96 1% )、CD4 0 (88 9% )、CD83(91 6 % )、HLA DR(92 9% )、HLA ABC(99% )、CD5 4 (97 5 % ) ,少量DC即可激发同种异体淋巴细胞增殖。结论 :为DC的进一步深入研究及临床应用奠定了良好的基础。Objective:Got high purity dendritic cells from PBMC recovered from one time used no leukocytes transfusion apparatus.Methods:Rinsed PBMC with natural salt water from one time used no leukocytes transfusion apparatus and induced PBMC with GM-CSF and IL-4.Results:Got 5.1×10 7 PBMC from every one time used no leukocytes transfusion apparatus and 4.7×10 6 DCs with a purity of 96.9% were obtained.These DCs express CD86(96.1%)?CD40(88.9%)?CD83(91.6%)?HLA-DR(92.9%)?HLA-ABC(99%)?CD54(97.5%) highly and a few number of these DCs could stimulate proliferation of allogeneic mixed leukocyte reactions strongly.Conclusion:The results of this research supplied basic data for further research of DC and its clinical application.

关 键 词：一次性滤出白细胞输血器 PBMC DC 

分 类 号：R392[医药卫生—免疫学]