高效液相色谱法测定中药枇杷叶中熊果酸的含量  

Determination of ursolic acid in Eriobotrya japonica by HPLC analysis

作　　者：鞠建华[1] 周亮[1] 郑瑞霞[1] 王春兰[1] 杨峻山[1] 

机构地区：[1]中国医学科学院中国协和医科大学药用植物研究所,北京100094

出　　处：《中国药学杂志》2003年第9期657-659,共3页Chinese Pharmaceutical Journal

基　　金：国家科技部重点科技项目 (攻关 )计划 ;中药现代化研究与产业化开发项目-常用中药化学成分及其有效成分的研究 (No .99-92 9-0 1-3 4)

摘　　要：目的　建立中药枇杷叶 [Eriobotryajaponica (Thunb .)Lindl.]中抗炎镇咳有效成分熊果酸的含量测定方法。 方法　No va pakC18(3.9mm× 15 0mm ,4 μm)色谱柱 ,流动相为乙腈 甲醇 水 醋酸胺 (6 0∶14∶2 8∶0 .6 ) ,流速为 1mL·min-1,检测波长为2 15nm。结果　熊果酸在 4 .2 2～ 9.85 μg范围内呈良好线性关系 ,回归方程为Y =5 .2 5× 10 5X +1.4 8× 10 5(r =0 .9996 ) ,平均回收率为 10 0 .3% ,RSD为 0 .0 2 %。结论　本方法简便 ,准确 ,重现性好 ,可作为枇杷叶的质量标准。OBJECTIVE: To establish an HPLC method for the determination of ursolic acid in Eriobotrya japonica (Thunb.) Lindl. METHODS: The determination was carried out with RP-HPLC on Nova-pak C18 column (3.9 mm × 150 mm, 4 μm) with acetonitril-methanol-water-ammonium acetate (60:14:28:0.6) as the mobile phase, and detected at the UV wavelength of 215 nm. RESULTS: The calibration curve was in good linearity over the range of 4.22∼9.85 μg with the regression equation Y = 5.25 × 105 X + 1.48 × 105(r = 0.9996) The average recovery was 100.3% with RSD = 0.02%. CONCLUSION: The method appeared to be simple, accurate, reproducible and can be used to control the quality of E. japonica.

关 键 词：高效液相色谱法 含量测定 中药 枇杷叶 熊果酸 

分 类 号：R286.0[医药卫生—中药学]