RP-HPLC测定血栓通注射液中4种皂苷的含量  

Quantitative Determination of Glycosides in Xueshuantong injection by RP-HPLC

作　　者：沈国芳[1] 吴永江[1] 程翼宇[1] 

机构地区：[1]浙江大学玉泉校区中国科学与工程学系,浙江杭州310027

出　　处：《中国药学杂志》2003年第9期698-699,共2页Chinese Pharmaceutical Journal

基　　金：国家重点基础研究发展规划 ( 973计划;G19990 5 44 0 5 );"十五"国家科技攻关计划重大项目 ( 2 0 0 1BA70 1A0 1)资助

摘　　要：目的　RP HPLC测定血栓通注射液中三七皂苷R1,人参皂苷Rg1,人参皂苷Rb1,人参皂苷Rd的含量。方法　采用Ag ilentSB C18分析柱 ,乙腈 磷酸 水线性梯度洗脱 ,检测波长 2 0 3nm。结果　该方法线性范围宽 ,加样回收率分别为R110 3.3% ,Rg110 1.8% ,Rb199.6 % ,Rd 10 3.9% ,方法重复性好 ,RSD <1.2 % (n =5 )。结论　建立的方法精密度高 ,结果准确可靠 ,重复性好 ,可用于血栓通注射液的质量控制。OBJECTIVE: To establish a RP-HPLC linear gradient elution method for simultaneously determining notoginsenoside R1, ginsenoside Rg 1, ginsenoside Rb1 and ginsenoside Rd in Xueshuantong injection. METHODS. An Agilent SB-C18 column (4.6 mm × 250 mm, 5 μm) was used with the mixture of acetonitrile-phosphoric acid-water as the mobile phase in linear gradient elution. The wavelength of detection was set at 203 nm. RESULTS: The response was linear over the wide concentration ranges. The average recoveries of R1, Rg1, Rb1 and Rd were 103.3%, 101.8%, 99.6% and Rd 103.9%, respectively. RSD of the assay was not more than 1.2% (n = 5). CONCLUSION: The method appeared to be accurate, sensitive and reliable, and can be used to control the quality of Xueshuantong injection.

关 键 词：RP-HPLC 含量测定 血栓通注射液 三七皂苷 人参皂苷 

分 类 号：R286.0[医药卫生—中药学]