用毛细管电泳拆分手性药物美索巴莫的方法研究  

Enantioseparation of methocarbamol by capillary zone electrophoresis using cyclodextrins as chiral additives

作　　者：黄志东[1] 张家明[1] 

机构地区：[1]天津市药品检验所,天津300070

出　　处：《中国药学杂志》2003年第9期700-702,共3页Chinese Pharmaceutical Journal

摘　　要：目的　采用 3种环糊精为毛细管电泳手性选择剂 ,研究美索巴莫对映体的拆分方法。方法　用区带毛细管电泳模式 ,探讨 β 环糊精、二甲基 β 环糊精和羧甲基 β 环糊精对美索巴莫对映体的拆分之影响 ,确定拆分美索巴莫对映体的最佳条件。此外 ,还探讨了手性识别机制。结果　以 β 环糊精和二甲基 β 环糊精作为手性选择剂时 ,不能使美索巴莫对映体达到基线分离 ,而羧甲基 β 环糊精能获得很好的分离结果。结论　在拆分碱性药物对映体时 ,可在低 pH下以酸性衍生化环糊精作为手性选择剂.OBJECTIVE: To investigate the enantioseparation of methocarbamol using capillary electrophoresis(CE) with three cyclodextrins as the chiral additives. METHODS: The enantioseparation of methocarbamol was processed with β-cyclodextrin(β-CD), dimethyl-β-cyclodextrin(DM-β-CD) and 2,6-di-O-carboxymethyl-β-cyclodextrin(CM-β-CD) as the chiral selectors. The effects of the pH, composition and concentration of the running buffer solution, the sort and concentration of chiral selectors, the running voltage, the capillary temperature on the mobility and chiral separation were studied respectively. In addition, the mechanism on chiral distinguish was discussed. RESULTS: CM-β-CD gave a baseline enantiomeric separation for the basic drugs, whereas the β-CD and DM-β-CD, under optimal conditions, showed poor stereoselectivity and gave just partial chiral separation. CONCLUSION: Lower pH of the running buffer solution tends to be more favorable for the enantioseparation of the basic drugs with acidic derivatized cyclodextrins.

关 键 词：毛细管电泳 药物拆分 手性药物 美索巴莫 环糊精 肌肉松弛剂 

分 类 号：R971.8[医药卫生—药品] R917[医药卫生—药学]