硫酸寡聚糖复合物对实验性腹主动脉瘤的抑制作用  

Inhibition of the formation of experimental abdominal aortic aneurysms by PI-88

作　　者：吴建秋[1] 景在平[1] 

机构地区：[1]第二军医大学附属长海医院血管外科,上海200433

出　　处：《中华普通外科杂志》2003年第9期537-539,共3页Chinese Journal of General Surgery

基　　金：国家自然科学基金资助项目 (3 9770 72 1)

摘　　要：目的观察乙酰肝素酶抑制剂PI 88对腹主动脉瘤形成的抑制作用。方法利用豚鼠—SD大鼠异种移植腹主动脉瘤模型 ,于移植术后 4周内连续PI 88治疗 ;术后 4周利用Northernblot杂交和免疫组化技术 ,检测移植腹主动脉直径、乙酰肝素酶表达、微血管增生及炎性细胞浸润程度。结果与非治疗组相比 ,治疗组腹主动脉乙酰肝素酶表达、移植血管直径、微血管增生及炎性细胞浸润程度均显著降低 ,但仍高于阴性对照组。结论PI 88可通过抑制乙酰肝素酶作用 ,控制腹主动脉瘤形成。Objective To evaluate heparanase inhibitor PI-88 in the inhibition of abdominal aortic aneurysm(AAA) formation. Methods A guinea pig-to-SD rat transplantation model of AAA was established for the observation of therapeutic effects of PI-88 given by continuously administration within 4 weeks after abdominal aortic transplantation. Abdominal aortic diameter, the degree of inflammatory infiltration, microvessel density and the expression of heparanase were determined by immunohistochemistry and Northern blot analysis. Results In contrast to the positive controls, the diameter and inflammatory cell infiltration of graft decreased remarkably after administrating PI-88, and the expression of heparanase and microvessel density decreased accordingly, although still upregulated when compared with negative control. Conclusions PI-88 could prevent formation of AAA by inhibiting heparanase activation.

关 键 词：硫酸寡聚糖复合物 实验 腹主动脉瘤 抑制作用 乙酰肝素酶 大鼠 

分 类 号：R654.4[医药卫生—外科学]