不同来源黄瓜遗传亲缘关系的RAPD分析  

RAPD Analysis and Taxology Discussion on Cucumber Materials from Different Fountainhead Areas

作　　者：刘殿林[1] 杨瑞环[1] 哈玉洁[1] 陈德富[2] 陈喜文[2] 夏立新[2] 

机构地区：[1]天津市黄瓜研究所,天津300192 [2]南开大学生物化学与分子生物学系,天津300071

出　　处：《华北农学报》2003年第3期50-52,共3页Acta Agriculturae Boreali-Sinica

基　　金：天津市重点攻关项目(993122511-2)

摘　　要：用RAPD技术对黄瓜基因组DNA进行多态性研究,分析了39份黄瓜材料的遗传差异。结果表明,所采用的172条随机引物中,有49条引物扩增谱带清晰且重复性较好,扩增总片段数达378个,单个引物的扩增片段数在4-12之间,片段大小在0.2-3.5 kb之间。不同材料间的遗传距离(D)在0.064-0.592之间,并根据遗传距离,按UWPGA法进行了聚类分析。Random amplified Polymorphic DNA(RAPD) was used to analyze the genetic diversity among cucumber inbred line. The genetic of 39 cucumber inbred line was studied. The result showed that 49 of 172 arbitrary primers could amplify clear and repeatable bands. 398 fragments were obtained. Each primer gave 4-12 bands. The length of the band was 0.2-3.5 kb. The genetic distance(D) value between different breeds of cucumber inbred line was 0.064-0.592. The D value was used to construct a dendrogram by UPGMA.

关 键 词：黄瓜 遗传差异 亲缘关系 RAPD分析 多态性 随机引物 遗传距离 遗传距离 聚类分析 

分 类 号：S642.2[农业科学—蔬菜学]