17β-雌二醇对神经干细胞分裂分化作用的观察  

Action of 17beta-estradiol on division and differentiation in rat neuralstem cell

作　　者：吴蕾[1] 胡祥友[1] 许浩[2] 王月菊[1] 秦松[2] 李嘉嘉[1] 周江宁[1] 

机构地区：[1]安徽医科大学第一附属医院老年病研究所,合肥230022 [2]中国科学技术大学生命科学院,合肥230027

出　　处：《安徽医科大学学报》2003年第5期329-333,共5页Acta Universitatis Medicinalis Anhui

基　　金：国家 973项目资助课题 (编号 :G19990 5 40 0 7)

摘　　要：目的　探讨雌激素对神经干细胞分裂分化的作用。方法　大鼠神经干细胞培养模型加入剂量为 10mg/L 17β 雌二醇和 10mmol/LBrdU ,分别在培养 4、12h取出细胞 ,进行免疫细胞化学标记。结果　培养 4h ,雌二醇组单位面积细胞总数和BrdU标记细胞与对照组比较差别无显著性 (P=0 2 5 9,P =0 0 6 7) ,培养 12h ,雌二醇组细胞BrdU胞核着色变浅 ,胞浆有着色 ,有 4 0 %的细胞可见明显突起 ,单位面积的细胞总数较对照组无显著性改变 (P =0 0 6 ) ,BrdU标记细胞数目减少 (P =0 0 0 3) ,提示分裂细胞减少。另一方面 ,培养 12h时的雌二醇处理组BrdU免疫荧光阳性细胞的光密度 (OD)显著高于对照组 (P <0 0 1)。结论　 17β 雌二醇可以抑制大鼠神经干细胞分裂 ,促进神经干细胞分化。Objective To detect the action of 17beta-est r adiol on division and differentiation of neural stem cells. Methods The neural stem cells derived from 12～12.5 d embryonic rat cortex were exposed to 17beta-estradiol (10 mg/L) and coincubated with BrdU to mark new DNA synthesis. Results No differences in the number of total cells and BrdU- immunopositive cells per unit was found between the estradiol and control group after 4h culture(P=0 259, P=0 067). However,after 12 h culture,Brd U-immunopositive cell number was decreased in the estradiol group,compared wit h the control (P=0 003). But the optical density of BrdU-immunopositive ce lls was increased in estradiol group (P<0 01). Also,40% of BrdU-immunopos itive cells with processes were found in estradiol group while only nucleus coul d be seen in the control. There was no difference in total cell number between t he two groups (P=0 06). Conclusion All these results sugge st that estradiol may inhibit division of neural stem cell and induce cell differentiation of morphology.oincubatedwithBrdUtomarknew

关 键 词：17Β-雌二醇 神经干细胞 细胞分裂 细胞分化 药理学 

分 类 号：R977.12[医药卫生—药品]