酵母酸性磷酸酯酶基因PHO81克隆的初步研究  

A PRELIMINARY STUDY ON CLONING OF YEAST ACID PHOSPHATASE GENE PHO 81

作　　者：王自春[1] 郝军山 

机构地区：[1]山西大学分子科学研究所

出　　处：《山西大学学报（自然科学版）》1992年第2期169-175,共7页Journal of Shanxi University(Natural Science Edition)

基　　金：山西省自然科学基金

摘　　要：酵母PHO81基因是调控阻遏酸性磷酸酯酶的的一种中介因子,其编码区由3531个核苷酸组成。由于片段较大,目前很难应用PCR技术一次予以扩增。本文用自行设计的4个引物以啤酒酵母总染色体DNA为模板借助PCR技术扩增PHO81基因的两个片段,其长度分别为1.3kb和2.2kb,通过限制性酶切将其装入载体质粒pUC18中。并初步进行了重组质粒限制性内切酶酶谱分析和Southern杂交试验。A complex genetic regulatory network controls the expression of the repressible acid phosphatase gene(PHO5)in yeast. According to the model proposed by Oshima, the PHO 81 gene plays a key role at the top of the hierarchy of at least 5 transacting regulatory genes. Under derepressive conditions the PHO 81 product interacts as a modulator with the negative controlling factors (products of PHO 85 and PHO 80). As a result the product of PHO 4 is released and combined with the product of PHO 2, thereby forming the activator complex of PHO 5 transcription.As the first step towards elucidating the molecular mechanisms involved in the expression and functional role of the PHO 81 gene,we have undertaken its molecular cloning and characterization.

关 键 词：PHO81基因 克隆 PCR 质粒pUC18 

分 类 号：O75[理学—晶体学]