镧系络合物的双向螯合剂——BCPDA的性质研究  

Study on the Properties of BCPDA

作　　者：潘利华[1] 曾春慧[1] 关铭[2] 赵超[1] 林敏[1] 谢文兵[1] 

机构地区：[1]中国科学院长春应用化学研究所,国家电化学光谱分析研究中心,稀土化学和物理开放实验室,吉林长春130022 [2]吉林大学药学院,吉林长春130021

出　　处：《光谱学与光谱分析》2003年第5期903-906,共4页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金(No.30070714)资助项目

摘　　要：对镧系络合物的双向螯合剂--4,7-二氯磺酰基苯基-1,10-菲咯啉-2,9-二羧酸(BCPDA)合成、表征和性质研究表明: 1. 通过IR光谱、 1H NMR、熔点、元素分析证明其结构正确. 2. BCPDA可溶于几种不同溶剂. 3. 几种体系的BCPDA溶液所得到的吸收光谱基本相同, 且BCPDA的浓度与吸光度在1.00～1.20×102 μmol*L-1范围内呈正比. 4. BCPDA标记蛋白质和铕离子与BCPDA螯合连接实验证实了BCPDA的双向功能.A new chelate-4, 7-bis (chlorosulfophenyl)-1,10-phenanthroline-2, 9-dicaboxylic acid (BCPDA) was synthesized for time-resolved fluorescence immunoassay (TRFIA). Its structure and purity were confirmed by means of IR, H-1 NMR, melt point and element analysis. Also, some of the BCPDA's properties, such as absorption spectra, emission spectra and fluorescence lifetime were discussed. A BCPDA-protein was formed when BCPDA reacted with protein under relative mild condition. A BCPDA-Eu3+ chelate was formed when BCPDA reacted with EuCl2 under certain condition. The BCPDA-Eu3+ can a-nit relatively strong fluorescence. The fluorescence lifetime of Eu3+-BCPDA-protein was measured as well.

关 键 词：镧系络合物 双向螯合剂 BCPDA 性质 时间分辨荧光免疫分析 

分 类 号：O614.33[理学—无机化学] O641.4[理学—化学]