荧光光度法测定鸟嘌呤  

Determination of Guanine by Spectrofluorimetry

作　　者：刘美林[1] 李保新[1] 许春丽[1] 章竹君[1] 

机构地区：[1]陕西师范大学化学与材料科学学院,陕西西安710062

出　　处：《光谱学与光谱分析》2003年第5期1012-1014,共3页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金项目(No.20175039);霍英东教育基金会项目(No.81013)资助

摘　　要：本文研究表明在碱性介质中,鸟嘌呤可被H2O2氧化(Cu2+作催化剂)生成8-羟基鸟嘌呤,该产物在397 nm处产生强的荧光。据此,建立了一种测定鸟嘌呤的新方法。鸟嘌呤的浓度在2.0×10^(-7)1.0×10^(-5)g·mL-1的范围内与荧光强度有良好的线性关系,检出限为8.0×10^(-8)g·mL-1。该方法简单、快速、灵敏度高,并成功地用于尿样中鸟嘌呤的测定。In base medium, it was found that guanine could be oxidized by hydrogen peroxide in the presence of Cu2+ as the catalyzer to produce 8-hydroyguanine, which possessed strong fluorescent characteristic at 397 nm. Based on this, a novel spectrofluorimetry for guanine was suggested. The linear range of calibration curve was 2.0 x 10(-7)-1.0 x 10(-5)g(.)mL(-1), and the detection limit was 8.0 x 10(-8)g(.)mL(-1).

关 键 词：荧光光度法 测定 鸟嘌呤 过氧化氢 碱性介质 药物 

分 类 号：TQ463.5[化学工程—制药化工] TQ460.72[医药卫生—药物分析学]