转基因鱼腥藻7120流加培养的研究  

Fed-batch Culture of Recombinant Anabaena sp. PCC7120

作　　者：张晨[1] 刘志伟[1] 郭勇[2] 

机构地区：[1]嘉应学院生物系,广东梅州514015 [2]华南理工大学食品与生物工程学院,广州510640

出　　处：《嘉应大学学报》2003年第3期44-46,共3页Journal of Jiaying University

基　　金：广东省自然科学基金(980541)

摘　　要：流加培养对转TNF—α基因鱼腥藻7120的生长有利.单独流加碳源对转基因鱼腥藻7120生长与外源基因表达影响很小,单独流加氮源和同时流加碳氮源可明显促进生长,生物量达到2.964g/L和3.059g/L,分别增长9.91％和12.71％,而且能轻微促进藻细胞中的TNF表达.Fed-batch culture was benefit for the growth of recombinant Anabaena sp. PCC7120. Feeding carbon source had little effect on the cell growth and exogenous gene expression. Feeding nitrogen source of feeding carbon and nitrogen source together could promote cell growth remarkably, and could increase TNF expression slightly. The biomass has increased 9. 91% and 12. 71% compared with batch culture respectively.

关 键 词：转基因鱼腥藻 肿瘤坏死因子 流加培养 

分 类 号：Q781[生物学—分子生物学]