微波消化-固相萃取光度法测定烟草中的铁  

Microwave Digestion and Solid Phase Extraction Spectrophotometric Determination of Iron in Tobacco

作　　者：孟冬玲[1] 杨国荣[1] 杨亚玲[1] 尹家元[2] 杨光宇[2] 

机构地区：[1]昆明理工大学生化学院,昆明650224 [2]云南大学化学系,昆明650091

出　　处：《云南化工》2003年第5期39-41,共3页Yunnan Chemical Technology

摘　　要：研究了用微波消化 ,α ,α’ 连吡啶固相萃取光度法测定烟草中的铁 ,烟草样品用微波消化后 ,消化液中的铁可在pH为 4.0的HAc NaAc缓冲介质中与α ,α’ 连吡啶反应生成 3∶1稳定络合物 ,该络合物可被C1 8固相萃取小柱萃取 ,小柱上富集的络合物用乙醇洗脱后用光度法测定 ,体系λmax =5 15nm ,ε =8.2 3× 10 3L·mol- 1 ·cm- 1 。铁含量在 0～ 5 μg/mL内符合比尔定律 。The color reaction of iron with dipyridyl was studied. In the presence of pH 4.0 buffer solutions, dipyridyl can react with iron(II) to form a stable 3:1 complex. This complex was enriched by solid phase extraction with C18 cartridge and eluted the retained chelate from cartridge by ethanol. The molar absorptivity is 8.23(103 L.mol-1.cm-1 at 515 nm. Beer's law is obeyed in range of 0-5 (g/mL. This method can be applied to the determination of iron in tobacco with satisfactory results.

关 键 词：微波消化—固相萃取光度法 测定 烟草 铁 α α′—连吡啶 

分 类 号：TS47[农业科学—烟草工业] O657.3[理学—分析化学]