葡萄总DNA提取方法的比较研究  

Comparison research of methods of DNA extraction from grape

作　　者：党尉[1] 尉亚辉[1] 张华平[1] 刘科[1] 

机构地区：[1]西北大学生命科学学院,陕西西安710069

出　　处：《西北大学学报（自然科学版）》2003年第5期572-574,602,共4页Journal of Northwest University（Natural Science Edition）

基　　金：陕西省自然科学基金资助项目(98SM02)

摘　　要：以葡萄幼叶为材料,分别采用溴化十六烷基三甲基铵(CTAB)法、高盐低pH法、简易法、高盐沉淀法和改良十二烷基硫酸钠(SDS)法5种方法提取总DNA,通过A260/A280值、琼脂糖凝胶电泳和RAPD对5种方法所得DNA溶液进行定量、定性分析和综合比较。结果显示高盐低pH法、高盐沉淀法综合效果较好,改良SDS法所得DNA纯度最佳。因此,在实际工作中还应根据实验目的选取最适合的方法。Five methods,i.e. CTAB,high saltlow pH,basic method,high salt precipitation, improved SDS method, were used to extract total DNA from the young leaves of grape. The resulted DNA samples of five methods were tested qualitatively and quantitatively using the value of A260/A280 、agarose gel electrophoresis and RAPD. The results show that high salt precipitation and high saltlow pH methods are better as a whole. The purity of extracted DNA by improve SDS is the best. So we should select the proper method according to the objetive in the study.

关 键 词：葡萄 总DNA(脱氧核糖核酸) 提取方法 聚合酶链反应 

分 类 号：Q503[生物学—生物化学]