血管内皮细胞生长因子对小鼠CFU-GM、CFU-E、CFU-S生成的影响  

The effects on murine CFU-GM、CFU-E、CFU-S formation by vascular endothelial growth factor

作　　者：仲照东[1] 胡中波[1] 刘凌波[1] 邹萍[1] 黄士昂[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院血液科,武汉430022

出　　处：《临床血液学杂志》2003年第6期277-279,共3页Journal of Clinical Hematology

基　　金：海外青年学者合作研究基金资助 (3992 80 10 )

摘　　要：目的 :研究血管内皮细胞生长因子 (VEGF)对小鼠骨髓单个核细胞 (MNC)粒 巨噬细胞系集落形成单位 (CFU GM)、红系集落形成单位 (CFU E)、脾集落形成单位 (CFU S)生成数量的影响。方法 :一部分小鼠骨髓MNC样本在加入或不加入外源性hVEGF的体系中预先培养 2 4h ,分别进行体外造血细胞集落培养和小鼠体内CFU S试验 ,计数CFU GM、CFU E、CFU S集落生成数目 ;另外对剩余骨髓样本不经处理直接在含rhVEGF的体系中进行体外CFU GM、CFU E集落培养 ,观察集落培养体系中添加VEGF对集落生成的影响。结果 :CFU GM、CFU E、CFU S数目分别为 33.2 1± 2 .84、72 .0 0± 4 .5 5和 12 .2 0± 1.32 ,显著高于未处理组 (分别为19.6 0± 2 .10、38.86± 2 .77和 6 .10± 1.5 2 ) (P <0 .0 5 ) ,但均低于正常对照组 (分别为 5 1.97± 2 .4 5、16 4 .2 0± 5 .70和 19.5 0± 2 .4 6 )。外源性hVEGF的存在使VEGF组CFU GM、CFU E产率较正常对照组显著提高 (73.12±3.80∶5 1.39± 2 .6 0 ,198.98± 4 .92∶16 4 .4 0± 5 .32 ) (P <0 .0 5 )。结论 :VEGF不仅对造血细胞集落生成能力具有保护作用 ,还具有促进造血细胞集落生成的作用。Objective:To study the effects on the colony-forming capacitity of hematopoietic cells by vascular endothelial growth factor.Method:First, BALB/c bone marrow hematopoietic cells(MNCs) were prepared. Some samples were pretreated with or without hVEGF for 24 hours and then used for CFU-GM、CFU-E、CFU-S assay. Second, Others were used for CFU-GM、CFU-E assay directly while hVEGF was also added into the culture system.Result:When BALB/c bone marrow MNCs were pretreated with hVEGF for 24 hours, the numbers of CFU-GM、CFU-E、CFU-S( 33.21± 2.84、 72.00± 4.55、 12.20± 1.32 )were significantly higher than those of untreated group( 19.60± 2.10、 38.86± 2.77、 6.10± 1.52)(P< 0.05). In the culture system of CFU-GM、CFU-E assays when fresh samples were used, the existence of hVEGF also promoted the number of CFU-GM、CFU-E( 73.12± 3.80 vs 51.39± 2.60, 198.98± 4.92 vs 164.40± 5.32)(P< 0.05).Conclusion:Not only can hVEGF sustain but also promote the colony-forming capacity of mice bone marrow mononuclear cells.

关 键 词：血管内皮细咆生长因子 造血细胞 骨髓 集落生成能力 

分 类 号：R331.1[医药卫生—人体生理学] R329.2[医药卫生—基础医学]