雌激素和流体剪切力对成骨细胞增殖和功能的影响  

Effects of estrogen and wall-shear stress on rat osteblasts in vitro

作　　者：曹玲[1] 丁寅[1] 

机构地区：[1]西安第四军医大学口腔医学院,710032

出　　处：《实用口腔医学杂志》2003年第5期475-478,共4页Journal of Practical Stomatology

摘　　要：目的 :初步了解雌激素与流体剪切力单独作用及联合作用对体外培养的成骨细胞增殖和功能的影响。方法 :采用酶消化法进行SD大鼠新生子鼠颅盖骨成骨细胞的原代培养 ,将生长活跃、特性稳定的III～IV代成骨细胞 ,用于实验。选择恒定的雌激素浓度及合适的振荡频率分 4种组合方式施加于成骨细胞上 ,比较这 4种方式在不同时间段对细胞增殖和碱性磷酸酶活性有何不同影响。结果 :雌激素和流体剪切力均可促进成骨细胞的增殖和ALP的活性 ,但长时间的剪切力作用则抑制细胞增殖。流体剪切力早期可迅速提高成骨细胞的ALP活性 ,比雌激素作用显著。雌激素和流体剪切力联合作用对成骨细胞的增殖和分化也有促进作用 ,但两者并非总是协同作用 ,在细胞分化早期对细胞增殖表现为协同作用 ,但对ALP活性却表现为拮抗作用。结论 :雌激素和流体剪切力及两者复合作用均可促进体外培养的成骨细胞增殖 。Objective: To observed the efects of estrogen and wall-shear stress alone or in combination on the proliferation and function of rat osteoblast in vitro. Methods: Isolated and purified osteoblast from the calvaria of newborn SD rats were cultured and passaged.The cells of passes three to four were treated with 0.1 nmol/L estrogen (goup E),wall-shear stress at 80 r/min (group WSS) or 0.1 nmol/L combined with wall-sher stress at 80 r/min (group EWSS) respectively.Cell proliferation was studied by MTT assay and alkline phosphatase (ALP ) by a ALP test kit. Results: Both the estrogen and wall-shear stress alone caused the increase of cells proliferation and alkaline phosphatase (ALP) activity.But long period of wall-shear stress decreased the cell proliferation.Wall-shear stress increased ALP activity more quickly and more remarkably than the estrogen did.The combination of estrogen and wall-shear stress increased the cell proliferation and the ALP activity.In the early stage (6～24 h) of the cell proliferation, the combination functioned synergicly.The combination functioned antagonistically on ALP from 6 to 12 h while synergicly after 12 h. Conclusion: Estrogen and wall-shear stress can elevate the cell proliferation, ALP activity of osteoblasts in vitro.

关 键 词：雌激素 流体剪切力 成骨细胞 细胞增殖 骨钙素 碱性磷酸酶 

分 类 号：R318.01[医药卫生—生物医学工程]