嗜酸性粒细胞阳离子蛋白测定的影响因素探讨  

Study of influence factors on the eosinophil cationic protein level determination

作　　者：温怀凯[1] 阮逸群[1] 

机构地区：[1]浙江省温州医学院附属第二医院检验科,325027

出　　处：《江西医学检验》2003年第5期327-328,共2页Jiangxi Journal of Medical Laboratory Sciences

摘　　要：目的探讨温度、抗凝剂(EDTA-K2)对血嗜酸性粒细胞阳离子蛋白(ECP)水平的影响。方法采用固相双位点酶放大化学发光法,分别对22例健康体检者在两种不同温度(25℃和37℃)的血清标本,以及在相同温度(37℃)下,加抗凝剂和不加抗凝剂的血标本进行ECP水平测定。结果25℃组和37℃组血清ECP水平分别为8.92±6.45μg/L(x±s)和19.87±12.16μg/L(x±s),两组有显著性差异(P<0.01);37℃温度下加抗凝剂和不加抗凝剂血标本的ECP水平分别为5.26±2.60μg/L(x±s)和19.87±12.16μg/L(x±s),两组也有显著性差异(P<0.001)。结论温度和抗凝剂(EDTA-K2)对血标本的ECP水平测定有不同程度的影响。Objective To study the eff ects of temperature and anticoagulant(ED-TA-K 2 )on the eosinophil cationic protein(ECP)level.Methods Using immobile do u-ble-site enzyme amplified chemiluminescence immunoassay,serum ECP levels of22healthy persons were determined at two different clotting temperature( 25℃and37℃),and in the condition of anticoagulating with EDTA-K 2 and without anticoagulant at the same temperature of37℃.Results The s erum ECP level at37℃was significantly higher(P<0.01)than that at25℃ .There was significant difference(P<0.001)between the samples with EDT A-K 2 and those without anticoagulant at37℃.Conclusion Temperature and antic oagulant(EDTA-K 2 )can affect the ECP level in different degrees.

关 键 词：嗜酸性粒细胞阳离子蛋白 测定 影响因素 温度 抗凝剂 血清 

分 类 号：R446.11[医药卫生—诊断学]