杀螺剂室内筛选实验方法标准化的研究 Ⅲ 实验钉螺饲养时间对杀螺效果的影响  

STUDIES ON STANDARDIZATION OF METHODS FOR SCREENING MOLLUSCICIDES IN LABORATORY Ⅲ. BREEDING TIME OF SNAILS TO INFLUENCE THE EVALUATION ON THE MOL-LUSCICIDAL EFFICACY

作　　者：戴建荣[1] 梁幼生[1] 张燕萍[1] 徐明[1] 李洪军[1] 朱荫昌[1] 

机构地区：[1]江苏省血吸虫病防治研究所,无锡214064

出　　处：《中国血吸虫病防治杂志》2003年第5期346-348,共3页Chinese Journal of Schistosomiasis Control

基　　金：江苏省地病协会(ID:X2114)

摘　　要：目的了解钉螺从现场采集后,在实验室饲养不同时间对杀螺效果的影响,为杀螺剂室内筛选标准化方法的制定提供依据.方法平行采集江苏南京和安徽铜陵2地钉螺,分别在室内按常规方法饲养1、6、11、16、21、31、61 d和91d后分别进行杀螺实验;氯硝柳胺用脱氯自来水配成有效浓度为1.0、0.5、0.25、0.125、0.062 5 mg/L和0.031 3 mg/L的药液;在室温25℃用浸杀法观察杀螺效果.结果室内饲养1-11d,1 mg/L氯硝柳胺浸泡钉螺24 h,钉螺死亡率均达100%,杀螺效果稳定,南京钉螺24 h LC50为0.094 7-0.133 9 mg/L,48 h为0.071 8-0.092 6 mg/L;铜陵钉螺24 hLC50为0.082 5-0.1039 mg/L,48 h为0.071 8-0.082 5 mg/L.饲养时间超过11d后,杀螺效果显著降低且不稳定:11d与16 d 1 mg/L氯硝柳胺浸泡钉螺24 h,杀螺效果差异有显著性(X2南京24 h=22.26.P＜0.01;X2铜陵24 h=5.19,P＜0.05).结论钉螺在室内饲养时间超过11d,钉螺对杀螺剂的敏感性显著降低,可影响杀螺效果的评价,故实验用于杀螺剂筛选的钉螺,应选择在室内饲养时间不超过11d的钉螺为宜.Objective To understand whether the breeding time of snails influences the evaluation on molluscicidal efficacy against Oncomelania snails so as to make a standardization of methods for screening molluscicides in laboratory. Methods Snails collected from the endemic areas in Nanjing and Tongling cities bred for 1,6,11,16,21.31,61 and 91 days respectively, and then were immersed in 1.000 0,0.500 0,0.250 0,0.125 0,0.062 5 and 0.031 3 mg/L solution of niclosamide for 24 and 48 hours at 25℃ in laboratory. Results One hundred percent killing rate was achieved in the groups of the snails bred for 1 - 11 days and immersed in 1.0 mg/L niclosamide for 24 hours. The LC_(50) concentrations of snails collected from Nanjing, Jiangsu Province and immersed for 24 hours varied from 0.094 7 to 0.133 9 mg/L, and for 48 hours from 0.071 8 to 0.092 6 mg/L.Those of snails collected from Tongling, Anhui Province for 24 hours varied from 0.082 5 to 0.103 9 mg/L, and for 48 hours from 0. 071 8 to 0.082 5 mg/L. The molluscicidal effect was unstable in the groups of snails bred for more than 16 days. There was a significant difference (X_(Nanjing24 h)~2 = 22.26,P<0.01 ;X_(Tongling24 h)~2 = 5.19,P<0.05)in molluscicidal effect of niclosamide between groups of snails bred for 11 and 16 days. Conclusion The breeding time of snails for screening molluscicides in laboratory should be shorter than 11 days.

关 键 词：钉螺 饲养时间 标准化 杀螺效果 氯硝柳胺 

分 类 号：R383.24[医药卫生—医学寄生虫学]