Enhancement of germ cell apoptosis induced by ethanol in transgenic mice overexpressing Fas Ligand  

作　　者：JIAHUAHU JIEJIANG YINGHUAMA NAYANG MAOHUZHANG MINWU JIANFEI LIHEGUO 

机构地区：[1]LaboratoryofMolecularCellBiology,InstituteofBiochemistryandCellBiology,ShanghaiInstitutesforBiologicalSciences,ChineseAcademyofSciences,Shanghai200031,China [2]Collegeoflifescience,ZhejiangUniversity,Hangzhou310027,China

出　　处：《Cell Research》2003年第5期361-368,共8页细胞研究（英文版）

基　　金：This work was supported by foundations from Chinese Academy of Sciences and Special Funds for Major State Basic Research of China(No.G19990539).

摘　　要：It was suggested that chronic ethanol exposure could result in testicular germ cell apoptosis, but the mechanism is still unclear. In the present study, we use a model of transgenic mice ubiquitously overexpressing human FasL to investigate whether Fas ligand plays a role in ethanol-induced testicular germ cell apoptosis. Both wild-type (WT)mice and transgenic (TG) mice were treated with acute ethanol (20% v/v) by introperitoneal injection for five times.After ethanol injection, WT mice displayed up-regulation of Fas ligand in the testes, which was shown by FITCconjugated flow cytometry and western blotting. Moreover, TG mice exhibited significantly more apoptotic germ cells than WT mice did after ethanol injection, which was demonstrated by DNA fragmentation, PI staining flow cytometry and TUNEL staining. In addition, histopathological examination revealed that degenerative changes of epithelial component of the tubules occurred in FasL overexpressing transgenic mice while testicular morphology was normal in wild-type mice after acute ethanol exposure, suggesting FasL expression determines the sensitivity of testes to ethanol in mice. In summary, we provide the direct evidences that Fas ligand mediates the apoptosis of testicular germ cells induced by acute ethanol using FasL transgenic mice.

关 键 词：Fas ligand ETHANOL APOPTOSIS TESTES transgenic mouse. 

分 类 号：Q786[生物学—分子生物学]