中华绒螯蟹苗期丝状细菌病病原HL-1的研究  

A pathogen HL-1 isolated from Eriocheir sinensis larvae with filamentous bacterial disease

作　　者：万夕和[1] 李筠[2] 张美如[1] 荆燕[1] 裴鸿生[1] 纪伟尚[2] 徐怀恕[2] 许璞[1] 

机构地区：[1]江苏省海洋水产研究所,江苏南通226007 [2]中国海洋大学海洋生命学院,山东青岛266003

出　　处：《中国水产科学》2003年第5期392-397,共6页Journal of Fishery Sciences of China

基　　金：江苏省科技厅应用基础研究项目(BJ-2000046)

摘　　要：从江苏省如东县中华绒螯蟹(Eriocheirsinensis)苗期暴发丝状细菌病的蚤状幼体分离得到丝状细菌HL 1,经感染试验证实其为中华绒螯蟹苗期丝状细菌病的病原。对HL 1的细胞形态、生理生化特征、致病性及药物敏感性研究结果表明,HL 1为无色、不分枝的丝状体,在固体培养基上形成指纹状菌落,菌丝可断裂形成微生子,在液体培养基中所形成的微生子可聚集成玫瑰花环样结构;HL 1过氧化氢酶阳性,几丁质酶阴性,需氧,鉴定为毛霉亮发菌(Leucothrixmucor),其生长的pH适宜范围为5.0～10.0,盐度最适范围为10～40;在常用的32种抗生素中,HL 1仅对乙基西羧霉素高度敏感,而对其他31种抗生素大多数具耐药性。The diseased Eriocheir sinensis larvae (zoea) were collected from three crab farms in Jiangsu Province during the outbreak period of filamentous bacterial disease in crab, and the filamentous bacterium strain HL1 was isolated from the zoea of E.sinensis with typical symptoms of filamentous bacterial disease. The observation was made under microscope and the results showed that the strain HL1 was colorless, unbranched and filamentous protokaryotic, and it could form a 'thumbprint' colony on solid substrates, and its gonidia could form 'rosette' in liquid substrates. Also, the strain was catalase positive, chitinase negative and aerobic. The optimum pH for the strain′s growth was 5.0-10.0, and the optimum salinity was 10-40. The strain HL1 was identified as Leucothrix mucor. The artificial infection results indicated that the L. mucor HL1 was the pathogen of the filamentous bacteria disease in E. sinensis larvae. The sensitivities of strain HL1 to 32 kinds of chemotherapeutants were tested that HL1 is highly sensitive to netilmicin, middly sensitive to ampicillin, carbenicillin, Penicillin G, streptomycin, neomycin, polymyxin B novobiocin and piperacillin , and unsensitive to the rest 23 kinds of chemotherapeutants.

关 键 词：中华绒螯蟹 苗期 丝状细菌病 毛霉亮发菌 耐药性 致病性 药敏试验 形态学特征 

分 类 号：S945[农业科学—水产养殖]