一种分离培养硫酸盐还原菌的改进方法  

A IMPROVED METHOD FOR ISLATION AND CULTIVATION OF SULFATE-REDUCING BACTERIA

作　　者：万海清[1] 苏仕军[1] 葛长海[1] 万雪松[1] 朱家骅[1] 

机构地区：[1]四川大学化工学院,成都610065

出　　处：《应用与环境生物学报》2003年第5期561-562,共2页Chinese Journal of Applied and Environmental Biology

基　　金：国家自然科学基金 (2 0 0 760 30 )项目资助~~

摘　　要：On the base of the characteristics of sulfate-reducing bacteria(SRB) growth and dish cultivated method, a new method for isolation and cultivation of SRB is established. It is dilution spread and repeat dish sandwish cultivated method with the exellence of dilution spread and embeded growth, and it can help separation and identification of SRB. The method has simple operation and less equipment. And using this method, the natural SRB drop was successfully gained, and small agar pieces containing SRB drop were acquired in easiness. The brief SRB transfering operation in strict anaerobic condition was indeed realized. Fig 1, RefOn the base of the characteristics of sulfate-reducing bacteria(SRB) growth and dish cultivated method, a new method for isolation and cultivation of SRB is established. It is dilution spread and repeat dish sandwish cultivated method with the exellence of dilution spread and embeded growth, and it can help separation and identification of SRB. The method has simple operation and less equipment. And using this method, the natural SRB drop was successfully gained, and small agar pieces containing SRB drop were acquired in easiness. The brief SRB transfering operation in strict anaerobic condition was indeed realized. Fig 1, Ref 7

关 键 词：厌氧菌 分离培养 稀释涂布 叠皿夹层 

分 类 号：Q933[生物学—微生物学]