尿液HIV-1抗体EIA-PRC法检测的临床应用  

Clinical Use of the Calypte^(TM) HIV-1 Urine EIA-PRC

作　　者：冯国基[1] 郑长青[1] 于剑[1] 杨林[1] 孙辉[1] 黄妍[1] 陈兰坤 

机构地区：[1]解放军第401医院输血科,山东青岛266071

出　　处：《现代检验医学杂志》2003年第5期16-17,共2页Journal of Modern Laboratory Medicine

摘　　要：目的 对尿液HIV-1抗体EIA试剂盒进行特异性与敏感性的评价。方法 收集尿液标本进行HIV-1尿液EIA-PRC法检测,对比血液标本进行HIV(1+2)抗体EIA初筛检查(双抗原夹心法)。结果 在5 000例所调查的正常人群中,有54例尿液HIV-1初检为阳性(54/5 000),复检为阴性结果。对48例AIDS患者的尿液标本进行尿液HIV-1检查,结果均为阳性(48/48)。结论 对尿液行HIV-1抗体的初筛检测,标本易得,且无创伤性,既减少了HIV交叉感染和传播的风险,又适宜于大批量的人群监测与普查工作,值得推广应用。Objective To evaluate the specificity and sensitivity of the Calypte?HIV-1 Urine EIA-PRC. Methods The specificity of CalypteTM HIV-1 Urine EIA-PRC was tested at one site which routinely performs HIV antibody determination on urine and serum specimens collected from healthy Blood donors. The sensitivity of Calypte?HIV-1 Urine EIA-PRC was evaluated by assaying 48 paired urine and serum specimens obtained from subjects with a clinical diagnosis of AIDS. Results When the urine EIA results were compared to the serum ELA results,in all five thousand(5000) urine specimens, 54 specimens tested were urine initially reactive and all repeated tests were negative. The specificity of Calypte橦IV-l Urine EIA-PRC in this study was estimated to be 98. 92% (4946/5000). In all 48 AIDS patients specimens,48 of the 48 urine specimens were repeatedly reactive. In this population the sensitivity of the Calypte?HIV-1 Urine EIA-PRC was 100% (48/48). Conclusion Detection of urine HIV-1 antibody is worthy developing to the clinic,especially facilitating surveillance and screening for HIV infection.

关 键 词：艾滋病 尿液 HIV抗体 酶联免疫法 

分 类 号：R446.62[医药卫生—诊断学] R512.91[医药卫生—临床医学]