免疫印迹技术检测抗ENA抗体谱的应用现状及技术进展  

Applied Status and Technical Improvement of Anti-ENA Autoantibody Profile Detection by Immunoblotting Technique

作　　者：兰庆萍[1] 

机构地区：[1]成都中医药大学附属医院检验科,四川成都610072

出　　处：《现代检验医学杂志》2003年第5期22-24,共3页Journal of Modern Laboratory Medicine

摘　　要：目的 分析探讨免疫印迹法(IBT)检测可提取核抗原(ENA)多肽抗体谱报告结果和临床应用中出现的常见问题、解决措施及技术进展。方法 利用万浮生物技术公司的IBT试剂盒检测抗ENA抗体进行观察,分析总结近年来该项检测结果出现的问题。结果 因为对抗ENA自身抗体谱的谱带特征认识不足,对免疫印迹法缺乏深入了解,在临床中出现抗Sm、抗U1RNAP、抗SSA、抗rRNP等抗体的错误判断;另一方面,临床医师过分地看重实验报告,以至为数不少的病人被误诊、误治,造成不可弥补的医源性疾患。结论 为了使免疫印迹法测定抗ENA抗体谱更好地运用于临床,不论检验人员还是风湿免疫科医师,均应正确看待多肽抗体检测的临床意义。Objective To analyze and find out the frequent problems and solvable measures involved in laboratory result and clinical application of the immunoblotting (IBT)detection anti-ENA polypeptide autoantibody profile,and study the improvement of this technology. Methods Detected ENA autoantibody profile by IBT assay kit which made of wanfo biotechnology CO. LTD,and analyzed the major problems emerged of the detective result in hospital recent years. Results Because of the unfamiliarity with the relevant knowledge concerning ENA autoantiboody profile as well as IBT technique,often made mistaken result involved in clinical laboratory test example:misjudgement of anti-Sm,anti-UIRNP,anti-SSA,anti-rRNP antiboodies,etc. On the other hand,some clinical doctors overemphasized laboratory report. It could make many patients are misdiagnose and mistherapy,so led up to many medical diseases which couldn't be recuperated. Conclusion To ensure the best clinical application by IBT technique detection ENA autoantibody profile. Not only laboratory workers but also rheumatic section doctors must treat correctly with detective of anti-ENA polypeptide antibody.

关 键 词：免疫印迹 抗ENA抗体 技术进展 

分 类 号：Q503[生物学—生物化学] R446.62[医药卫生—诊断学]