广东省三市致倦库蚊种群的抗性水平、非特异性酯酶分布频率及遗传分化  

Resistant Level,Frequency of Non-specific Esterases and Genetic Differentiation in Mosquitoes Culex pipiens quinquefasciatus in Three Cities of Guangdong

作　　者：张柯[1] 叶镇清[1] 乔传令[1] 林立丰[2] 蔡松武[2] 

机构地区：[1]中国科学院动物研究所农业虫鼠害综合治理研究国家重点实验室,北京100080 [2]广东省卫生防疫站,广东广州510000

出　　处：《Zoological Research》2003年第5期367-372,共6页动物学研究（英文）

基　　金：国家自然科学基金资助项目 ( 3 9980 0 3 4)

摘　　要：致卷库蚊 (Culexpipiensquinquefasciatus)样本于 2 0 0 1年 5月采自广州、佛山和中山。利用生物测定和淀粉凝胶电泳检测其对有机磷和氨基甲酸酯类杀虫剂的抗性水平和抗性相关酯酶的分布频率。与北京敏感品系相比 ,3个种群对敌敌畏、对硫磷、仲丁威的抗性水平分别至少为 2 1、 8和 5倍 ;以连锁形式存在的A8/B8和A9/B9是 3种群主要的高活性酯酶 ,但A2 /B2和B1有地区差异。由于种群间的基因流动 ,这些差异行将消失。Samples of mosquitoes Culex pipiens quinquefasciatus were collected in Guangzhou,Foshan,Zhongshan in Guangdong Province in May 2001.Bioassay and starch gel electrophoresis were used to determine their resistant levels to organophosphates and BPMC,and the distribution frequency of resistant-related esterases in the 3 populations.The resistant level of the 3 populations to dichlorovos was higher than the sensitive strain of Beijing at least for 21 times,parathion for 9 times,and BPMC for 5 times.The associated A8/B8 and A9/B9 were the main highly active esterases in the 3 populations,but regional differences appeared in esterase A2/B2 and B1.It was supposed that the difference will disappeared due to gene flow.

关 键 词：致倦库蚊 有机磷杀虫剂 酯酶 遗传分化 

分 类 号：R384[医药卫生—医学寄生虫学]