青阳参组织培养及愈伤组织的成分分析(英文)  

Studies on the Tissue Culture of Cynanchum otophyllum and Calli Chemical Constituents

作　　者：赵沛基[1] 甘烦远[1] 珠娜[1] 沈月毛[1] 

机构地区：[1]中国科学院昆明植物研究所,昆明650204

出　　处：《植物学通报》2003年第5期565-571,共7页Chinese Bulletin of Botany

基　　金：国家自然科学基金 (3 0 0 70 0 0 7);云南省科学基金 (99B0 0 1 7G)资助项目

摘　　要：用青阳参 (Cynanchumotophyllum)的嫩枝和芽在MS + 2 .0mg/L 2 ,4_D + 0 .1mg/LKIN的培养基上诱导愈伤组织。通过不同的培养基和激素配比实验 ,发现 6,7_V + 2 .0mg/L 2 ,4_D + 0 .3mg/LKIN最适合愈伤组织的生长。但在 6,7_V + 1 .0mg/L 2 ,4_D + 0 .1mg/LKIN培养基中的愈伤组织次生代谢物含量最高。愈伤组织的生长周期为 2 7d ,但在 33d时次生代谢产物的含量最高。从愈伤组织中分离到 7个化合物 :⑴ 9,1 0 ,1 1_三羟基_十八碳_1 2 (Z)_烯酸甲酯 (methyl 9,1 0 ,1 1_trihydroxy_1 2_octadecenoate) ,( 2 )胡萝卜甙 (daucosterol) ,( 3) β 谷甾醇 ( β sitosterol) ,( 4 )华木酸 (betulinicacid) ,( 5 )齐端果酸 (oleanolicacid) ,( 6)棕榈酸 (hexadecanoicacid) ,( 7)十八碳_9_烯酸 ( 9_octadecenoicacid)。首次报道从植物愈伤组织中分离到多羟基十八碳烯酸 ,并讨论了化合物 ( 1 )The calli of Cynanchum otophyllum were induced from the tender stems and young buds on MS medium with the supplement of 2.0 mg/L 2,4_D and 0.1 mg/L KIN. The result showed that 6,7_V medium was the optimal medium for callus growth. The tests using plant growth regulators with various concentrations showed that 6,7_V medium plus 2.0 mg/L 2,4_D and 0.3 mg/L KIN was the best combination for callus growth, whereas the production of secondary metabolites was higher on 6,7_V medium plus 1.0 mg/L 2,4_D and 0.1 mg/L KIN. The best period of culture was 27 d for callus growth, and 33 d for the maximal production of secondary metabolites. The isolation of nature products from the callus provided seven compounds including (1) fatty acid methyl ester named methyl 9,10,11_trihydroxy_12_octadecenoate and (2) daucosterol, (3) β sitosterol, (4) betulinic acid, (5) oleanolic acid, (6) hexadecanoic acid, (7) 9_octadecenoic acid. This is the first report of the isolation of poly_hydroxyl octadecenoic acid from a callus, and its proven biological function on the growth of plant cells was discussed.

关 键 词：青阳参 组织培养 愈伤组织 成分分析 次生代谢产物 

分 类 号：Q943[生物学—植物学]