都可喜对慢性间断性缺氧大鼠学习记忆能力及脑内单胺类神经递质的影响  

Effect of Duxil on learning-memory ability and brain monoaminergic neurotransmitter in chronic episodic hypoxia rat

作　　者：徐雁[1] 李舜伟[1] 张建军[2] 张毅[2] 

机构地区：[1]中国医学科学院 中国协和医科大学 北京协和医院神经科,北京100730 [2]中国医学科学院 中国协和医科大学药物研究所,北京100050

出　　处：《中国新药杂志》2003年第10期828-831,共4页Chinese Journal of New Drugs

摘　　要：目的:探讨都可喜(Duxil,阿米三嗪+萝巴新)对慢性间断性缺氧(EHYP)大鼠学习记忆能力和脑内单胺类神经递质水平的影响。方法:建立EHYP大鼠模型,并给予Duxil(0.03片·350g^(-1)体重,bid)干预。用被动避暗回避反射试验评价大鼠学习记忆能力,潜伏期(STL)越长,学习记忆能力越强;用高效液相色谱电化学检测器法测定大鼠皮层、海马和纹状体内去甲肾上腺素(NE)、多巴胺(DA)和5-羟色胺(5-HT)等单胺类神经递质的含量。结果:与对照组相比,EHYP组大鼠STL明显缩短(P<0.01),各脑区单胺类神经递质水平显著降低(P<0.05)。与EHYP组相比,Duxil组大鼠STL显著延长(P<0.01),皮层NE和DA含量、海马NE,DA和5-HT含量以及纹状体NE,DA和5-HT含量显著升高(P<0.05)。结论:Duxil可改善EHYP大鼠学习记忆能力并提高脑内单胺类神经递质水平。Objective:To study the effect of Duxil (almitrine+ raubasine) on learning-memory ability and brain monoaminergic neurotransmitter level in chronic episodic hypoxia (EHYP) rat. Meth- ods:After establishment of EHYP rat model,Duxil (0.03tablets·350g-1 ,bid) was given to the EHYP rat. The learning-memory ability was evaluated by the passive avoidance test and the levels of monoaminergic neurotransmitters, including norepinephrine (NE), dopamine (DA) and serotonin (5-HT) , were determined in three different brain regions (including cerebral cortex, hippocampus and straitum) by high performance liquid chromatography and electrochemical detection (HPLC-ECD). Results:Compared with the controlled rats, the performance on passive avoidance test of EHYP rats impaired significantly (P<0.01) and their levels of monoaminergic neurotransmitters in all different brain regions decreased significantly (P < 0. 05). Compared with EHYP rats, the rats treated with Duxil showed better performance on passive avoidance test (P< 0. 01) and higher level of brain monoaminergic neurotransmitters (except 5-HT level in cerebral cortex, P < 0. 05). Conclusion: Duxil can improve learning-memory ability and increase brain monoaminergic neurotransmitter in EHYP rats.

关 键 词：都可喜 慢性间断性缺氧 被动避暗回避反射试验 去甲肾上腺素 多巴胺 5-羟色胺 

分 类 号：R971.7[医药卫生—药品] R965.1[医药卫生—药学]