短暂脑缺血发作对溶血磷脂酸的影响及养血清脑颗粒的作用  

The effect of transient ischemic attach (TIA) on lysophosphatidic acid(LPA) and the influence of Yangxueqingnao granule on it

作　　者：牛建平[1] 薛萍[2] 伍期专[1] 李丹[1] 姚存姗[1] 王燕华[1] 包鹤丘[1] 

机构地区：[1]北京大学第一医院神经内科,北京100034 [2]铁道部北京铁路总医院干部医疗科,北京100048

出　　处：《中国新药杂志》2003年第10期831-833,共3页Chinese Journal of New Drugs

摘　　要：目的:研究短暂性脑缺血发作(TIA)对溶血磷脂酸(LPA)的影响以及中成药养血清脑颗粒的作用。方法:小鼠尾静脉注射过氧化物并窒息建立TIA模型,灌胃给予不同剂量养血清脑颗粒,诱发4次后隔日断头取脑测定血浆和脑组织中LPA等的含量。结果:模型组的神经系统评分为0.969±0.790,养血清脑颗粒2g·kg^(-1)组的评分为0.500±0.408,较模型组显著降低(P<0.05)。模型组小鼠血浆LPA比正常对照组明显升高,分别为(6.12±0.35)和(2.11±1.27)μmol·L^(-1),养血清脑颗粒4g·kg^(-1)组较模型组明显降低[(4.88±1.55)μmol·L^(-1),P<0.05]。模型组小鼠脑组织磷脂与对照相比明显降低,分别为(2.27±1.13)及(6.58±2.77)μmol·g^(-1),给药组小鼠可上升至(4.26±1.99)μmol·g^(-1)。结论:实验性TIA发作可导致小鼠血浆和脑组织中LPA等磷脂水平异常,养血清脑有逆转作用。Objective:To study the effect of TIA on LPA and the influence of Yangxueqingnao granule on it. Methods: Mice TIA model was established by injection of peroxide. Yangxueqingnao granule was given by gastric infusion once daily for 4d and the brain and plasma level of LPA were determined. Results: The stroke score was (0. 500 ± 0. 408) for Yangxueqingnao granule group (2g · kg -1) and (0. 969 ± 0. 790) for model group, and there was significant difference between the two groups. The plasma LPA was (6.12 ± 0. 35)μmol·L -1 for model group, (2. 11 ± 1. 27)μmol· L-1 for normal group and (4.48 ± 1. 55)μmol·L-1 for Yangxueqingnao granule group (4g·kg-1), there were significant difference between groups ( P < 0. 05 ) . The brain LPA level of the model group was significantly lower than that of the normal group[(2.27 ± 1.13) vs (6.58 ± 2. 77)μmol·g-1 ,P<0.05] ,and that of Yangxueqingnao granule group (4g · kg ) markedly increased in comparison with that of the model group [(4.26± 1.99) vs (2.27 ± 1. 13)μmol·g-1, P<0.05]. Conclusion: The LPA changes induced by TIA can be reversed by Yangxueqingnao granule.

关 键 词：养血清脑颗粒 短暂性脑缺血发作 溶血磷脂酸 

分 类 号：R288.5[医药卫生—中药学] R289.5[医药卫生—中医学]