小鼠骨骼肌细胞核DNA降解与死亡时间的关系  

A study of the relationship between the postmortem interval and nuclear DNA degradation of muscle cells in mice

作　　者：邓立彬[1] 余纯应[1] 杨庆恩[1] 

机构地区：[1]华中科技大学同济医学院法医物证教研室,湖北武汉430030

出　　处：《中国法医学杂志》2003年第5期273-275,共3页Chinese Journal of Forensic Medicine

摘　　要：目的　监测小鼠死后骨骼肌核内DNA降解的情况 ,探讨死后细胞核DNA降解的一般规律。方法　建立小鼠死亡模型 ,在死后 72h内 ,以 12h的间隔取骨骼肌样本进行单细胞凝胶电泳 ,在荧光显微镜下测量彗星图像 ,并作统计学分析。结果　机体死后 ,骨骼肌细胞在电泳图像上出现了明显的彗星形拖尾 ,L/W比值随死亡时间而逐渐增大 ,二者呈一定的线性关系。结论　单细胞凝胶电泳技术可以应用于早期死亡时间推断。Objective To evaluate the regular degradation of nuclear DNA of muscle cells in mice after death. Method A mouse death model was established. Skeletal muscle was sampled at 12-hour-interval within 72 hours after death. After single-cell gel electrophoresis, the comet-length and comet-width were scaled under fluorescent microscope. Results An evident comet tailing was observed in DNA of muscle cells after electrophoresis. The ratio of L/W increased with the extension of postmortem interval, which was correlated linearly with PMI from 0 to 48h postmortem. Conclusion DNA degradation by single-cell gel electrophoresis may be used as a predictor of PMI.

关 键 词：小鼠 骨骼肌细胞核 DNA降解 死亡时间 法医病理学 单细胞凝胶电泳 

分 类 号：D919[医药卫生—法医学]