小鼠皮肤切创愈合过程中caspase-6、-7表达的免疫组织化学研究  

An immunohistochemical study on the expressions of caspase-6、-7 during the skin incised wound healing in mice

作　　者：杜宇[1] 官大威[1] 赵锐[1] 

机构地区：[1]中国医科大学法医学院法医病理学教研室,辽宁沈阳110001

出　　处：《中国法医学杂志》2003年第5期259-261,共3页Chinese Journal of Forensic Medicine

基　　金：国家自然科学基金 (编号 3 0 2 713 47);教育部留学归国基金 (教外留司 [2 0 0 2 ] 3 67)资助项目

摘　　要：目的　观察皮肤切创损伤愈合过程中 ,caspase 6、 7在损伤周边区内的表达及其变化规律。方法　应用免疫组织化学技术观察伤后不同时间小鼠皮肤切创组织中caspase 6、 7的表达 ,以非切创小鼠皮肤组织作对照。 结果　伤后 6h的损伤皮肤组织中可见少量多核粒细胞表达caspase 6、 7,伤后 12～ 2 4h ,大部分浸润的多核粒细胞及部分单核细胞为caspase 6、 7阳性。随伤后时间延长 ,caspase 6、 7阳性细胞以单核细胞及成纤维细胞为主。伤后 0～ 3h ,caspase 6、 7阳性细胞比率较低 ,12h后逐渐增加 ,伤后 3d达高峰 ,其后逐渐下降。 结论　小鼠皮肤损伤愈合过程中 ,caspase 6、 7在损伤周边区内中性粒细胞 ,单核细胞及成纤维细胞中表达 ,其时序性变化规律可望用于皮肤损伤时间的推断。Objective To investigate the expressions of caspase-6,-7 during skin wound healing and the applicability of the time-dependent expressions of caspase-6,-7 to determination of the wound age.Method The expressions of caspase-6,-7 were studied in skin incised wound in mice by immunohistochemical SABC method,and the non-incised mouse skin was used as control.Results Expressions of caspase-6,-7 were detectable in polymorphonuclear cells (PMNs) in the wound specimens aged 6h. In the wound specimens aged from 12h to 24h after injury, caspase-6,-7 were identified in a large number of infiltrating PMNs and part of mononuclear cells (MNCs). Afterwards, the MNCs and fibroblastic cells (FBCs) accounted for the most part of the caspase-6,-7-positive cells. Morphometrically, the ratios of the number of the caspase-6,-7-stained PMNs, MNCs and FBCs to total number of the cells in the wounds were evaluated and calculated. The ratios of the caspase-6,-7-positive cells were low in the wound specimens aged from 0h to 3h, and maximized in the wound specimens aged 3 day. Thereafter, the ratios decreased and minimized in the specimens aged 14 day.Conclusion The results suggest that caspase-6,-7 were expressed in neutrophils, macrophages and fibroblasts during healing process of skin incised wound in mice. Caspase-6,-7 may be used as a marker for the wound age determination.

关 键 词：小鼠 CASPASE-6 表达 免疫组织化学 CASPASE-7 皮肤损伤 损伤时间推断 

分 类 号：D919[医药卫生—法医学]