乙型肝炎病毒宫内感染的分子流行病学研究  

A molecular epidemiological study on the intrauterine transmission of hepatitis B

作　　者：张振开 刘巍[2] 黄少新 邬质彬[3] 

机构地区：[1]广西桂林市疾病预防控制中心,541001 [2]广西疾病预防控制中心,530021 [3]广西医科大学公共卫生学院

出　　处：《广西预防医学》2003年第5期264-267,共4页Guangxi Journal of Preventive Medicine

摘　　要：目的为了掌握乙型肝炎病毒宫内感染的发生率和危险因素,探讨HBV相关抗体在胎儿体内产生的作用。方法用ELISA法筛选出HBsAg阳性孕妇及引产胎儿153例 ,HBsAg阴性孕妇及胎儿65例 ,共218例。然后用多聚酶链技术对孕妇 -引产胎儿HBV宫内感染进行检测。结果HBsAg阳性孕妇HBVDNA宫内感染率为53.59 %(82/153) ,HBsAg阴性孕妇静脉血中6.15%(4/65)检出HBVDNA阳性 ,两者相比有显著性差异 (P<0.01) ;母亲血中HBsAg、HBeAg、抗 -HBc同时阳性其相应胎儿HBVDNA感染率为92.5%(74/80) ,46例抗 -HBs阳性的胎儿血有5例检出HBVDNA阳性。结论HBV宫内感染率较高 ,HBsAg阴性孕妇也可能发生HBV宫内感染 。Objective To investigate the hepatitis B virus(HBV)intrauterine infection rate and risk factors.Methods Polymerese Chain Reaction(PCR)was used to detect the serum HBV DNA of the218pregnant womeninduced abortion foetuses.Results The heart blood HBV DNA positive rates of fetuses from HBsAg positive mothers were53.59%(82/153),out of65HBsAg nocarrier pregnant women,four were found HBV DNA positive in their fetuse heart blood(6.15%),there were signifiˉcant differences between the two groups(P<0.01).The heart blood HBV DNA positive rate of feˉtuses from HBsAg,HBeAg and Anti_HBc positive mothers was92.50%(74/80).There were5foeˉtuse heart blood HBVDNA positive in the46Anti_HBs positive fetuses(8.70%).Conclusion HBV intrauterine infection was a important mode of HBV transission,The HBVMof HBsAg,HBeAg and Anti_HBc positiveness is a risk factor of HBV intrauterine transmission.There is no protective acˉtion of positive Anti_HBs in foetuses.

关 键 词：乙型肝炎病毒 宫内感染 分子流行病学 危险因素 ELISA 

分 类 号：R714.251[医药卫生—妇产科学]