胸膜肺炎放线杆菌apxⅡ C突变株转移载体pSKPG的构建  

Construction of the Transfer Vector pSKPG for a Vaccine Strain of Actinobacillus Pleuropneumoniae by Mutagenesis of the apxⅡ C Gene

作　　者：徐晓娟[1] 何启盖[1] 陈焕春[1] 徐高原[1] 

机构地区：[1]华中农业大学动物医学院,湖北武汉430070

出　　处：《中国兽医学报》2003年第6期551-554,共4页Chinese Journal of Veterinary Science

基　　金：湖北省重点科技攻关项目 (2 0 0 1AA2 0 1B0 2 ) ;国家自然科学基金资助项目(3 0 2 0 0 11)

摘　　要：通过 PCR方法克隆了胸膜肺炎放线杆菌 ( Actinobacillus pleuropneum oniae)武汉株完整的 apx C基因和部分apx A基因约 3.4 kb。将该 DNA片段克隆到载体 p Bluescript SK( + )中得到质粒 p SKPP,并进一步将 gfp基因插入到 apx C基因的 Xba1 位点处 ,构建了 apx C基因插入失活的转移载体 p SKPG,从而为构建胸膜肺炎放线杆菌apxIn the study,the 3 4 kb DNA fragment including apxⅡC gene and part of apxⅡC gene was amplified by PCR and cloned into the plasmid pBluscriptⅡ SK+,and the recombinant plasmid was denominated pSKPP.The gfp gene was inserted into Xba1Ⅰ site of pSKPP,and the Xba1Ⅰ site locats in the apxⅡC gene.So the transfer vector pSKPG was constructed.This work will lay a basis for further research on candidate vaccine strain against Actinobacillus pleuropneumoniae.

关 键 词：胸膜肺炎放线杆菌 猪 传染性胸膜肺炎 apxⅡC基因 转移载体 毒力基因 基因改造 

分 类 号：S858.28[农业科学—临床兽医学]