獐牙菜属及近缘植物中齐墩果酸的高效液相色谱定量分析  

Quantitative analysis of oleanolic acid in Swertia genus and relative plants by HPLC

作　　者：陈家春[1] 周青[2] 左毅[2] 李惠[2] 刘焱文[2] 贾敏如[1] 

机构地区：[1]成都中医药大学,四川成都610075 [2]湖北中医学院,湖北武汉430061

出　　处：《中国医院药学杂志》2003年第11期641-643,共3页Chinese Journal of Hospital Pharmacy

基　　金：国家自然科学基金(项目批准号 30271590)

摘　　要：目的:比较獐牙菜属及近缘植物中齐墩果酸的含量,为资源利用提供依据。方法:高效液相色谱法测定獐牙菜属及其近缘植物不同部位中的齐墩果酸含量,Shim-Pack VP-ODS(5 μm,4.6 mm×150 mm)柱,流动相:乙腈、水(78:22),检测波长215 nm,0.05 AUFS,流速1.0 mL·min^(-1),柱温40℃。结果:测定贵州獐牙菜中齐墩果酸的平均回收率为102.1％,重现性实验的RSD为1.15％,符合定量分析要求。分别测定了14种獐牙菜属及近缘植物不同部位齐墩果酸的含量。14种植物花、叶、茎中均含齐墩果酸,其中花中的含量最高,从0.937％到1.386％;叶与茎中的含量其次,且含量差异很大,从0.236％到1.041％;根中的含量为零。结论:本次实验说明这些在民间作为獐牙菜使用的植物中都含有保肝成分齐墩果酸,且在花中的含量明显高于其他部位。OBJECTIVE To analyze and compare the content of oleanolic acid in 12 species of Swertia and it's related plants and to supply a basis for the resources exploitation. METHODS Detecting the content of Oleanolic acid in Suiertia and it's related plants by HPLC. The Shim-Pack VP-ODS(5 μm,4.6 mm×150 mm)column was used. The mobile phase consisted of CH3CN-Water (78:22) with the detection wavelength at 215 nm,0.05 AUFS. The flow rates was 1.0 mL·min -1. The temperature of column was 40℃. RESULTS The mean recovery of oleanolic acid in Swertia kouitchensis was 102.1 % . The RSD was 1.15%, which met the requirement of quantitative analysis. Therefore, different parts of 14 species of Swertia and related plant were tested respectively. There were oleanolic acid in all these 14 kinds of plants flowers, leaves and stems, in which the content of oleanolic acid was the highest in flower, ranging from 0.937% to 1.386% . The content of oleanolic acid in leaves and stems was next to that in flower, and the difference was notable, ranging from 0.236% to 1.041 % . There wasn' t oleanolic acid in roots. CONCLUSIONS This experiment all these plants used for Zhangyacai in folk medicine contain the constituent for protecting liver, namely oleanilic acid and the Content of oleanolic acid in flowers was higher than that in other parts of the plants.

关 键 词：獐牙菜属 齐墩果酸 高效液相色谱法 

分 类 号：R965.1[医药卫生—药理学]