反相高效液相色谱法测定双嘧达莫的血药浓度  

Determination of dipyridamol concentration in serum by HPLC

作　　者：黄雪梅[1] 项光剑[1] 郭瑰春[2] 吴禧明[2] 

机构地区：[1]广西壮族自治区人民医院,广西南宁530021 [2]桂林医学院,实习生广西桂林541004

出　　处：《中国医院药学杂志》2003年第11期668-669,共2页Chinese Journal of Hospital Pharmacy

摘　　要：目的:建立人血清中双嘧达莫的浓度测定方法。方法:采用高效液相色谱法,色谱柱:大连Nova-Pak C_(18)(4 μm,150mm×4.0mm);柱温:40℃;流动相为甲醇-0.1％磷酸氢二钠(75:25);流速:0.8 mL·min^(-1);检测波长:285 nm。结果:双嘧达莫血药浓度在0.483～61.76 mg·L^(-1)范围内线性良好,相关系数为0.9999,最小检测浓度为0.193 g·L^(-1);测得方法回收率及提取回收率分别为95.41％～106.75％和78.72％～89.97％,日内和日间RSD均小于7％。结论:本方法简便、准确,适合于双嘧达莫的血药浓度监测及药动学研究。OBJECTIVE To establish an HPLC method for the determination of dipyridamole concentration in serum. METHODS Nova-Pak C18 column was used with methanol-0.1 % sodium phosphate dibasic anhydrous (75 :25) as mobile phase, the flow rate was 0.8 mL·min-1,detection wavelength was 285 nm, temperature was 40℃. RESULTS The linear rang of serum concentration was 0.483 - 61.76 mg·L -1 ( r = 0.9999), detection was 0.193 mg·L-1. The relative and extractive recoveries were 95.41 % ~ 106.75 % and 78.72 % ~ 89.97 % . The RSD for inter-day and intra-day were all less than 7% . CONCLUSIONS The method is simple and accurate, and suitable for the determination of dipyridamole in serum and its pharmacodynamics.

关 键 词：双嘧达莫 高效液相色谱法 血药浓度 

分 类 号：R927.2[医药卫生—药学]