细胞因子对人类胚胎干细胞分离克隆的影响  

Effects of cytokines on isolation and cloning of human embryonic stem-like cells

作　　者：李松[1,2] 窦忠英 宋文刚[1] 华进联 张显忠[1] 张彬彬[1] 

机构地区：[1]泰山医学院免疫学教研室 [2]陕西省干细胞研究中心,陕西杨凌712100 [3]陕西省干细胞研究中心

出　　处：《泰山医学院学报》2003年第2期91-92,共2页Journal of Taishan Medical College

基　　金：国家自然科学基金 (39970 36 3);国家 973项目资助(G19990 5 430 0 )

摘　　要：目的　观察白血病抑制因子、干细胞因子、碱性成纤维细胞生长因子对人类胚胎干细胞分离克隆的影响。方法　在基础细胞培养液中加入不同的细胞因子组合 ,培养后观察人类胚胎干细胞集落形成数和各组的最高传代数。结果　细胞因子添加与否对人类胚胎干细胞原代培养无显著的影响 ,而对后继培养有显著影响。结论　在人类胚胎干细胞初代培养时可以不添加细胞因子 ,细胞的克隆过程中添加细胞因子有利于细胞的生长。Objective: To investigate the effects of the cytokines on isolation and cloning of human embryonic stem-like cells.Methods: The different combination of cytokines were mixed with the culture medium of cells .After a period, the cloning number of human embryonic stem-like cells and the topmost passage were observed and registered. Results: There was not obvious effect on the primary culture of human ESC but the effect was distinct on the later passage if cytokines were used. Conclusion: In the primary passage , human ESC may survive without cytokines, and the impact of the existence of cytokines is better on the cloning of human ESC than that of its nonexistence.

关 键 词：细胞因子 人类胚胎干细胞 分离克隆 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]