中药复方对辐射损伤小鼠脾细胞凋亡及mRNA表达的影响  

Effect of Digan oral liquid on apoptosis and expression of mRNA of some apoptosis-related genes in the spleen tissue of mice with radiation injury

作　　者：潘琦[1] 何东初[2] 吴江平[2] 陈如泉[3] 黄星原[1] 

机构地区：[1]武汉大学人民医院,430060 [2]广州军区武汉总医院 [3]湖北中医学院附属医院

出　　处：《中华放射医学与防护杂志》2003年第5期341-342,共2页Chinese Journal of Radiological Medicine and Protection

基　　金：湖北省自然科学基金资助项目 (97J0 80 )

摘　　要：目的　探讨中药复方地甘口服液 (DGOL)对辐射损伤小鼠脾细胞凋亡及凋亡相关基因bcl 2和baxmRNA表达的作用。方法　建立辐射损伤模型 (一次性 7 0Gy的X射线照射 ) ,喂饲10 0 %地甘口服液每次 0 2ml 10g小鼠 ,每日 2次。第 10天用原位末端标记的TUNEL技术检测脾组织细胞凋亡和原位杂交法检测凋亡相关基因bcl 2和baxmRNA的表达。结果　地甘口服液组与对照组比脾脏的细胞凋亡率和baxmRNA表达显著减少 (P <0 0 1) ,bcl 2mRNA的表达显著增强 (P <0 0 1)。结论　中药复方地甘口服液可能通过促进bcl 2和降低baxmRNA的表达 ,促进造血细胞的增生 ,提高机体免疫力。Objective To study the effect of Digan oral liquid(DGOL) on apoptosis and expression of mRNA of some apoptosis-related genes in the spleen tissue of mice with radiation injury.Methods After irradiation with 7.5 Gy X- rays by a linear accelerator ,the animals were fed with or without 0.2 ml/10 g of 100% DGOL.Apoptosis was detected by terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling(TUNEL) in situ hybridiation.Results The apoptotic ratio and bax-2 mRNA expression were significantly decreased ( P <0.01) in spleen tissue ,whereas bcl-2 mRNA expression was significantly increased ( P <0.01) in spleen tissue of the DGOL group than those in the control group. Conclusion DGOL can restrain the apoptosis in spleen tissue of mice with radiation injury, promote proliferation of hemopoietic cells , and elevate immunological function of the organism via enlancing bcl-2 expression and decreasing bax-2 mRNA expression. These results provide theoretical basis for clinical application of DGOL. ;

关 键 词：中药复方 辐射损伤 小鼠 脾细胞凋亡 MRNA 表达 影响 基因表达 地甘口服液 

分 类 号：R285[医药卫生—中药学]