黄曲霉毒素G_1对体外培养HPBM增殖及TNF-α分泌的影响  

Effects of aflatoxin G_1 on proliferation and TNF-α secretion of human peripheral blood mononuclear cells in vitro

作　　者：王会艳[1] 张祥宏[1] 严霞[1] 王俊灵[1] 孙旭明[1] 杨永滨[1] 王凤荣[1] 

机构地区：[1]河北医科大学基础所病理室,河北石家庄050017

出　　处：《中国病理生理杂志》2001年第2期131-133,共3页Chinese Journal of Pathophysiology

基　　金：河北省自然科学基金资助项目 (No 3 972 5 3 )

摘　　要：目的 :研究黄曲霉毒素G1(AFG1)对体外培养人外周血单个核细胞 (HPBM)增殖及细胞TNF -α分泌的影响。方法 :采用流式细胞术 (FCM)和MTT比色法研究AFG1对HPBM增殖的影响。以双抗体夹心ELISA法检测AFG1对HPBMTNF -α分泌的影响。结果 :FCM检测结果显示 ,AFG1作用 6h ,10 0 0 μg/L处理组HPBM的增殖指数明显高于对照组。AFG1作用 2 4h ,2 0 0 μg/L和 10 0 0 μg/L浓度的AFG1可明显刺激HPBM增殖 ;回归分析结果表明 ,AFG1作用 6h和 2 4h ,AFG1浓度均与增殖指数呈正相关 (r分别为 0 5 12 2和 0 5 119,P均 <0 0 5 )。MTT比色法结果显示 ,2 0 0 0 μg/LAFG1处理HPBM的A值明显高于对照组。AFG1在 10 0 μg/L浓度下可显著抑制TNF -α分泌 (P <0 0 5 )。结论 :AFG1对体外培养HPBM的增殖有刺激作用 ,在 10 0 μg/L浓度下对HPBMTNF -α的分泌有一定抑制作用。AIM: To explore the effects of aflatoxin G 1(AFG 1 )on proliferation and TNF-α secretion of human peripheral blood mononuclear cells(HPBM) in vitro. METHODS: The effects of AFG 1 on proliferation of HPBM were analysed with flow cytometric (FCM) DNA analysis and MTT bioassay, while that on TNF-α secretion was detected with ELISA. RESULTS: FCM analysis revealed that 6 h after treatment, proliferation index(PI) of 1000 μg/L AFG 1 treated HPBM was significantly higher than that of control. 24 h after AFG 1 treatment, stimulating effects on proliferation was found in HPBM treated with AFG 1 at 200 μg/L and 1 000 μg/L.Regression analysis showed that PI was postively correlated with the concentrations of AFG 1 in the concentration range from 0 to 1 000 μg/L( r=0. 5122 and 0.5119 respectively,P<0.05).MTT bioassay showed that the A value of the cells treated with AFG 1 at 2 000 μg/L was higher than that of the control. Double antibody sandwich enzyme linked immunosorbent assay (ELISA) results showed that AFG 1 at a dose of 100 μg/L could significantly inhibit lipopolysaccharide-induced TNF-α secretion. CONCLUSION: AFG 1 could stimulate the proliferation of HPBM and could decrease TNF-α secretion at certain concentration.

关 键 词：单核细胞 淋巴细胞 黄曲霉毒素类 DNA 肿瘤坏死因子 

分 类 号：Q253[生物学—细胞生物学]