长期摄取高钠盐饮食引发的高血压发病机制实验研究  

Experimental study on pathogenesis of salt-induced hypertension

作　　者：符云峰[1] 孙纪新[2] 李素琴[1] 卢振敏[1] 孙爱励[2] 张北奇[3] 

机构地区：[1]河北省医学科学院实验医学研究所生化研究室,河北石家庄050021 [2]河北省医学科学院分析测试中心,河北石家庄050021 [3]河北省医学科学院病理生理研究室,河北石家庄050021

出　　处：《中国病理生理杂志》2001年第2期143-146,共4页Chinese Journal of Pathophysiology

基　　金：河北省自然科学基金资助项目 (No .3 993 91)

摘　　要：目的 :探讨高钠盐引发的高血压发病机制。方法 :雄性SD大鼠 4 0只 ,分为对照 (NC)组、高盐 (HS)组、高盐 +L -精氨酸 (HS +Arg)组、高盐 +依那普利 (HS +En)组和高盐 +特拉唑嗪 (HS +Ter)组 ,每组 8只。各组饲料相同。NC组饮去离子水 ;HS组饮 1 5 %氯化钠溶液 ;HS +Arg组、HS +En组和HS +Ter组分别饮用 1 5 %氯化钠溶液配制的L -精氨酸 ( 4g·kg-1·d-1)、依那普利 ( 3 0mg·kg-1·d-1)和盐酸特拉唑嗪 ( 4mg·kg-1·d-1)。第 8周末 ,大鼠在戊巴比妥钠麻醉下 ,直接测量颈总动脉血压 ,开腹抽取下腔静脉血及剖取肾脏、肾上腺。测定前海葱苷原A样物质 (PLC)水平 ,Na+ K+ ATP酶活性 ,NO(x)、内皮素 (ET)及血管紧张素II(AngII)水平。结果 :HS组大鼠血压显著高于NC组 ,血浆PLC、ET及肾上腺AngII水平皆显著高于NC组 ;血浆和肾组织NO(x)和AngII水平、肾脏Na+ K+ ATP酶活性皆明显低于NC组 ;HS +Arg组、HS +En组和HS +Ter组大鼠血压和血浆PLC水平皆显著低于HS组 ,血浆和肾组织NO(x)水平及Na+ K+ ATP酶活性皆明显高于HS组 ;HS +Arg组、HS +En组和HS +Ter组肾上腺AngII水平及HS +Arg组血浆ET水平明显低于HS组 ,并与NC组相近。结论 :在高钠盐引发的高血压发病机制中 ,除钠泵抑制因子释放增加致细胞膜Na+ K+ ATP酶活性?AIM: To reveal the pathogenesis of salt-induced hypertension. METHODS: Forty male SD rats were divided into five groups, which received on same chow but different drink. Control(NC)group: deionized water; High salt(HS)group: 1.5% NaCl solution; L-arginine(HS+Arg)group: L-arginine(4 g·kg -1 ·d -1 )in 1.5% NaCl solution; Enalapril (HS+En) group: enalapril (30 mg·kg -1 ·d -1 ) in 1.5% NaCl solution; Terazozin(HS+Ter)group: terazozin(4 mg·kg -1 ·d -1 )in 1.5% NaCl solution. At the end of 8 weeks, rats were anesthetized with pentobarbital sodium. Blood pressure(BP)were recorded and blood were drawn from inferior vena cava and kidneys, adrenals were removed. NO(x),ET and AngII, Na + K + ATPase and proscillaridin-like compound(PLC)were assayed. RESULTS: BP, PLC and ET in plasma and AngII in adrenal were increased, NO(x)and AngII in plasma and kidney were decreased in HS group compared with NC group. CONCLUSION: High salt intake may induce hypertension in SD rats. In addition to the Na + K + ATPase activity was inhibited by increased sodium-pump inhibitors, NO release decrease may also play an important role in the pathogenesis of hypertension.

关 键 词：氯化钠 钠 膳食 腺苷三磷酸酶 钠 钾 一氧化氮 高血压 

分 类 号：R544.1[医药卫生—心血管疾病]