锌-金属硫蛋白对严重烫伤大鼠氧自由基损伤的保护作用  

The protective effects of Zn metallothionein on severely scalded rats inflicted by oxygen free radicals

作　　者：陈旭[1] 覃凤均[1] 程时[2] 孙永华[1] 

机构地区：[1]北京积水潭医院烧伤科,100035 [2]北京大学医学部生物物理系

出　　处：《中华烧伤杂志》2001年第1期46-48,共3页Chinese Journal of Burns

摘　　要：目的 研究严重烫伤大鼠延迟复苏后氧自由基损伤及锌金属硫蛋白(MT)的保护作用。 方法 采用大鼠30%TBSAⅢ度烫伤模型，分成正常对照组、延迟复苏组、MT治疗组、维生素C(VitC)治疗组，应用电子自旋共振(ESR)技术和传统间接检测手段，观察伤后24、48 h血浆及烧伤水肿液中超氧化物歧化酶(SOD)、丙二醛（MDA）的变化，同时对心、肝、肾、小肠的组织形态及血浆生化指标进行检测。 结果 延迟复苏组血SOD含量下降，血及水肿液MDA明显升高，各脏器组织形态及血生化指标发生明显变化。MT治疗组较延迟复苏组SOD增高（P<0.05），MDA含量明显下降（P<0.05），脏器组织形态及血生化指标改善，且优于VitC治疗组。 结论 严重烫伤大鼠延迟复苏后存在氧自由基损伤，应用锌-金属硫蛋白治疗有一定保护作用。Objective To investigate the protective effects of metallothionein(MT) on severely scalded rats inflicted by oxygen free radicals after delayed resuscitation. Methods Wistar rats inflicted by 30% TBSAⅢdegree scalding injury were taken as the model.Fifty Six rats were randomly divided into four groups,i.e.normal control,delayed resuscitation,MT treated and VitC treated groups.The changes in superoxide dismutase(SOD) and malondialdehyde(MDA) contents in plasma and subeschar edematous fluid were determined at 24 and 48 postburn hours with traditional indirect detecting method and electron spin resonance (ESR).Pathomorphological examination was simultaneously carried out for cardiac,hepatic,renal and intestinal samples.Plasma biochemical indices were determined at the same time. Results In delayed resuscitation group,SOD content decreased and the MDA contents increased in plasma and subeschar fluid obviously,with remarkable changes in the pathomorphology of all the internal organs and the blood biochemical indices.But in MT treating group,SOD content increased (P<0.05)with decreased content of MDA (P<0.05) and the pathomorphology and blood biochemical indices improved significantly when compared with those in delayed resusitation group and VitC treatment group. Conclusion MT treatment might be beneficial in the management of severely scalding rats infliced by oxygen free radicals.

关 键 词：锌-金属硫蛋白 严重烫伤 大鼠 氧自由基损伤 保护作用 超氧化物歧化酶 

分 类 号：R644[医药卫生—外科学]