中药814对实验性地鼠肺气肿及丙二醛、α_1-抗胰蛋白酶的影响  

The effects of Chinse medicine 814 on emphysema and contents of MDA α1-At in BALF in hamsters

作　　者：宋润兰[1] 阮英茆[1] 朱明娟[2] 陈样银 韩晓男[1] 韵慧枫[1] 张旭晨[1] 

机构地区：[1]中国医学科学院阜外心血管病医院等,北京100037 [2]中国医学科学院血液学研究所 [3]中国医学科学院基础医学研究所

出　　处：《中国心血管杂志》2000年第1期9-12,共4页Chinese Journal of Cardiovascular Medicine

摘　　要：目的 观察中药814对弹性蛋白酶致肺气肿的防治作用.并寻找其作用途径.方法 雄性金黄地鼠90只,分正常对照(生理盐水)组、肺气肿模型组、814用药组,于支气管灌注弹性蛋白酶制作肺气肿模型,于灌注酶前两周给予中药制剂814,4g/100gBW溶于生理盐水灌胃.动物分别于注酶后的一月、两月、三月处死,用显微镜微机测定肺泡平均内村间隔(MLI)、平均肺泡数(MAN)等项指标,观察肺气肿的病变程度.同时作气管肺泡灌洗液中丙二醛含量、血清中α_1-抗胰蛋白酶(α_1-AT))的测定.结果814防治组肺气肿各项指标都表现不同程度的缓解,丙二醛含量在肺气肿组有显著增加.而在814防治组较肺气肿组明显下降;α_1-AT在肺气肿组降低.在814防治组则有所回升.结论814可部分地阻抑弹性蛋白酶地鼠肺气肿的发生发展.从本实验推测814的防治作用与抑制氧自由基的产生、保护或减少α_1-AT丢失有关.Objective To invest the effects of Chinese medicine 814 on elastaese-induced emphysema in hamsters and to find the related factors of pathogenesis. Method emphysema models were set up by intratracheal instillation of e-lastase, 814 was administered from two weeks before instillation to 1,2.3 mondh later. After animals were killed, analysis for lung tissue section was made by the Microscopy-computor image analysis system : number (MAN、 MIL) were tested as the parameter to emtphysema. In the meamtime,the content of MDA in branch alveolar lavege liquid and α1-AT in serum were examined. Restults Treatment of 814 group showed less emphysema, and MDA contents in BALF in emphysema group higher than one in the normal control group, and treatment goup of 814 showed lower MDA in BALF. Compared with the normal control α1-AT in serum lower in emphysema group. and higher in 814 of month group than one in empyusema group. Conclusion 814 could partly inhibit the elastase-induced emphysema. and MDA and α1-AT are likely to be the related factors.

关 键 词：肺气肿 氧自由基 Α1-抗胰蛋白酶 实验 中药814 

分 类 号：R285[医药卫生—中药学]