微量法测定产气荚膜梭菌α毒素  

The Detection for α-tox in in Clostridium Perfringens by Micro-quantity Assay

作　　者：吕存女[1] 张敬友 赵红[1] 傅思武[1] 孟筱琦[1] 

机构地区：[1]卫生部兰州生物制品研究所梭菌专业实验室,甘肃兰州730046 [2]江苏省南京市动植物检疫局,江苏南京210009

出　　处：《兰州大学学报（自然科学版）》2000年第6期101-104,共4页Journal of Lanzhou University(Natural Sciences)

摘　　要：为了快速、灵敏地测定样品中的产气荚膜梭菌α毒素，本实验以产气荚膜梭菌培养滤 液经硫酸铵分段盐析、丙酮分段沉淀及凝胶过滤得精制α毒素.在96孔细胞板上，经卵黄反 应浊度(微量)法测定精制α毒素及培养滤液卵黄反应滴度分别为1∶32 768和1∶2 048，且 该方法特异性强、稳定性好，是产气荚膜梭菌α毒素的一种快速、简便的检测手段.A rapid and sensitive method has been used to detect α-toxin from Cl o stridium perfringens estimated in samples. In this study, the preparation of α - toxin was prepared by ammonium sulfate fractionation, acetone fractionation and gel filtration from the culture filtrate of clostridium perfringens. Egg units of α-toxin and culture filtrate were 218 453 EU/mg a nd 1 044 EU/mg respectively by measuring the turbidity of lecitho-vitelling soluti on in the microl iter plate. The results demonstrate that the method is rapid, easy , specific and stable to detect α-toxin of clostridium perfringens.

关 键 词：产气荚膜梭菌 Α毒素 卵黄反应浊度(微量) 

分 类 号：Q93-331[生物学—微生物学]