人神经生长抑制因子的单克隆抗体制备及抗原决定簇的研究  

Preparation of Neuronal Growth Inhibitory Factor Monoclonal Antibody and Studies of Its Epitope

作　　者：林芃[1] 季清洲[2] 任宏伟[2] 茹炳根[2] 

机构地区：[1]大连大学化学与化学工程系 辽宁大连 116622 [2]北京大学生命科学学院蛋白质工程国家重点实验室

出　　处：《大连大学学报》2001年第2期77-81,共5页Journal of Dalian University

摘　　要：神经生长抑制因子（ＦＩＦ）是一种特异存在于哺乳动物脑中的金鹰硫蛋白 （ＭＴ），又称ＭＴ－Ⅲ，具有特异神经细胞生长抑制效应．ＭＴ－Ⅲ与ＭＴ有７０％的序列 同源性，但ＭＴ却无此功能．在患ＡＤ症病人的大脑中，ＧＩＦ和ｍＲＮＡ的含量均显著降 低．为了进一步研究，ＭＴ－Ⅲ的结构与功能及与ＡＤ症的关系，制图示ＧＩＦ的单克隆抗 体，用ＥＬＩＳＡ方法研究了ＧＩＦ，α－结构域和β－结构域，Ｎ－末端六肽和Ｎ－末端＋肽分别 与ＧＩＦ的单克隆抗体的相互作用，并对单克隆抗体的抗原决定簇进行了分析．结果显示 了ＧＩＦ的抗原决定簇与其一定的空间构象有密切的关系．Neuronal growth inhibitory factor (GIF), a brain-specific metallothionein protein (Metallothionein- Ⅲ), can inhibit the growth of neuronal cell. Though exhibiting the 70% same sequence, GIF distinguishes from othet MT isoforms by inhibiting neuronal survival in vitro. It was reported that MT- Ⅲ and its mRNA were greatly reduced in Alzheimer's disease (AD) brain. For further research, the monoclonal antibody of GIF was prepared and the interaction between GIF, a-domain. β-domain, peptides and McAb of GIF were studied with ELISA. The epitope of the MT- Ⅲ was studied. The results show that the epitope of GIF is closely related to GIF's conformation.

关 键 词：神经生长抑制因子 GIF 单克隆抗体 抗原决定簇 金属硫蛋白 MT 中枢神经系统 

分 类 号：R338.2[医药卫生—人体生理学]