黑暗微好氧条件下浑球红假单胞菌的固氮生长  

Diazotrophic Growth of Rhodobacter sphaeroides under Microaerobic Conditions in the Dark

作　　者：杨大庆[1] 俞吉安[1] 朱章玉[1] 

机构地区：[1]上海交通大学生物技术研究所

出　　处：《上海交通大学学报》1992年第4期88-93,共6页Journal of Shanghai Jiaotong University

摘　　要：液体培养和深层琼脂固体培养,都表明浑球红假单胞菌在黑暗微好氧条件下能够依靠固定 N_2生长.在光照和黑暗有氧条件下,菌株的乙炔还原活性都能出现,但在光照情况下细胞固氮酶比黑暗时对氧更敏感.进一步实验表明,高浓度氧气不但抑制固氮酶的活性水平,而且使固氮酶活性的出现延迟.根据上述结果,讨论了浑球红假单胞菌的固氮作用与菌体的呼吸代谢及环境氧分压的相互关系.Diazotrophy of R.sphaeroides is not obligatorily linked to photosyn-thesis.In the dark,the capacity of growing for R.sphaeroides with N_2 as solenitrogen surce was demonstrated in deep agar shake cultures.R.sphaeroides canalso synthesize nitrogenase and fix dinitrogen under microaerobic conditions inliquid culture.Acetylene reduction by whole cells is more sensitive to oxygen inthe light than in the dark,and nitrogenase activity appears later and lower withthe presence of higher O_2 content(5%—20%)under either of the conditions.

关 键 词：固氮作用 呼吸 光合细菌 假单胞菌 

分 类 号：Q939.112[生物学—微生物学]