丰产鲫细菌性败血病的研究I——病原分离与鉴定  

STUDY ON THE BACTERIAL SEPTICEMIA OF CARASSIUS AURATUS OF PENZE(♀)× CYPRINUS ACUTIDORSALIS(♂)I——ISOLATION AND IDENTIFICATION OF THE PATHOGEN

作　　者：李小波[1] 黄文芳[1] 

机构地区：[1]华南师范大学生命科学学院

出　　处：《微生物学通报》2003年第5期56-60,共5页Microbiology China

摘　　要：从患败血病的丰产鲫 (Carassiusauratusofpenze (♀ )×Cyprinusacutidorsalis (♂ ) )脾脏中分离到的一株细菌CSS 4 2 ,经人工感染证实为病原菌。其主要特征为短杆状 ,革兰氏阴性 ,有运动性 ,极端生单鞭毛 ,不产生芽孢 ,无荚膜 ;对 0 1 2 9弧菌抑制剂不敏感 ,能利用葡萄糖、麦芽糖、甘露醇、蔗糖、肌醇等 ,不利用丙二酸盐、乳糖、木糖、棉子糖、山梨糖、福寿草醇等 ;氧化酶、精氨酸双水解酶阳性 ,尿素、苯丙氨酸脱羧酶、鸟氨酸脱羧酶阴性。经细菌自动化鉴定系统VITEK AMS 60鉴定 ,CSS 4 2菌株为豚鼠气单胞菌(Aeromonascaviae)。药物敏感试验表明 ,CSS 4 2对链霉素、妥布霉素、氯霉素、利福平、诺氟沙星、呋喃妥因等药物敏感 。The bacterial strain CSS-4-2 isolated from the spleen of Carassius auratus of penze(♀) × Cyprinus acutidorsalis (♂) with septicemia was proved to b e a pathogen by artificial infection. This bacteria has short rod-shaped cells , Gram negative and motile, with single polar flagellum, and with not capsule. No spore was formed , and it was resistant to 0/129. It could utilize glucose, mal t ose ,mannite, sucrose and inositol, but could not utilize malonate, lactose, xyl ose, raffinose, sorbin and adonitol. Oxidase and arginine dihydrolase was posit ive.While urease, lysine decarboxylase and ornthine decarboxylase was negative .Bacteriological identification by VITEK-AMS-60 showed the strain CSS-4-2 w as Aeromonas caviae. Drug sensitivity assay showed that this strain was sen sitive to s treptomycin, tobramycin, chloromycetin, rifampicin, norfloxacin and nitrofuranto in, but resistant to penicillin G.

关 键 词：丰产鲫 豚鼠气单胞菌 病原 鉴定 

分 类 号：S941[农业科学—水产养殖]