HIV/AIDS患者特异性细胞毒性T细胞功能的研究  

Study on specific cytotoxic T lymphocyte responses in HIV/AIDS patients

作　　者：尚红[1] 韩晓旭[1] 王亚男[1] 周立平[1] 张子宁[1] 姜拥军[1] 张民[1] 于旭 Bruce Walker 

机构地区：[1]中国医科大学附属第一医院艾滋病研究室,沈阳110001 [2]Partners AIDS Research Center and Infectious Disease Unit,Massachusetts General Hospital and Harvard Medical School

出　　处：《中华微生物学和免疫学杂志》2003年第9期696-699,共4页Chinese Journal of Microbiology and Immunology

基　　金：国家重大基础研究规划严重传染病防治基础研究资助项目 (G19990 5 410 7)

摘　　要：目的　了解中国HIV AIDS患者HIV特异性细胞毒性T细胞 (CTL)功能。方法　将覆盖HIV 1P15、P17和P2 4Gag全长的 94个重叠多肽作为抗原 ,用IFN γELISPOT方法检测HIV AIDS患者HIV 1特异性CTL功能。结果　HIV 1抗原多肽P17 15、P17 16、P2 4 7、P17 8,P2 4 2 8最易被HIV AIDS患者特异性CTL识别。HIV感染者识别HIV 1多肽的数量和强度均高于AIDS患者。结论　我国HIV AIDS患者体内存在识别不同HIV 1Gag多肽的特异性CTL ,且HIV特异性CTL功能与疾病进展相关。Objective To investigate the HIV specific cytotoxic T lymphocyte response in HIV/AIDS patients of China. Methods A panel of 94 overlapping peptides, spanning the entire P17 Gag, P24 Gag, P15 Gag were used as antigen. We examined the HIV-1 specific cytotoxic T lymphocyte responses of Chinese HIV/AIDS patients by IFN-γ ELISPOT assay. Results HIV-1 antigen peptides P17-15, P17-16, P24-7, P17-8 and P24-28 were more frequently recognized by the HIV-1 specific cytotoxic T lymphocyte. The magnitude and breadth of the CTL responses were much higher in HIV carriers than those in AIDS patients. Conclusion There were HIV-1 specific cytotoxic T lymphocyte responses directly against different HIV-1 Gag peptides in Chinese HIV/AIDS patients, and the responses were correlated with disease progress.

关 键 词：HIV/AIDS患者 特异性 细胞毒性T细胞 功能 研究 艾滋病 

分 类 号：R285[医药卫生—中药学]