板蓝根抑制脂多糖诱导的p38蛋白激酶活性研究  

Study on inhibitory effect of Radix-Isatidis on endotoxin-induced p38 MAPK activity

作　　者：林剑国 刘云海[2] 程晓红 石淑仙[2] 

机构地区：[1]武汉市传染病医院,430022 [2]华中科技大学同济医学院附属同济医院

出　　处：《中华微生物学和免疫学杂志》2003年第9期739-741,共3页Chinese Journal of Microbiology and Immunology

基　　金：国家自然科学基金 (3 9870 872 ) ;卫生部科学研究基金(98 2 110 )资助项目

摘　　要：目的　探讨板蓝根对内毒素介导的信号传导的影响。方法　取出BALB c小鼠腹腔内的单核细胞 ,分别用板蓝根提取液Ⅲ组分 (1mg ml) +LPS 10 0 μg ml;板蓝根提取液Ⅲ组分 (1mg ml) +金黄色葡萄球菌 (10 6 CFU ml) ;只用LPS 10 0 μg ml;不加LPS和其它药物 ,直接用DMEM培养液 ,培养 6h。取培养上清液检测TNF、IL 6、NO水平 ,收集细胞检测胞内p38MAPK(丝裂原活化蛋白激酶 )活性。然后将板蓝根液按倍比稀释至 1∶8倍时 ,加入LPS 10 0 μg ml,培养 6h后收集细胞测p38MAPK活性。结果　加入板蓝根提取液的单核细胞p38MAPK活性未见明显增强 ,TNF、IL 6、NO水平无明显上升 ,与单独加入LPS后这些指标显著增高相比 ,差异有极显著性 (P <0 .0 1)。而加入金黄色葡萄球菌组单个核细胞p38MAPK活性 ,产生TNF、IL 6、NO浓度仍然较高。板蓝根液倍比稀释后 ,p38MAPK活性逐渐上升。结论　板蓝根可特异性抑制由内毒素介导的p38活性 ,并呈浓度依赖性。Objective To investigate the effect of Radix-Isatidis on endotoxin-mediated signaling pathway. Methods Peritoneal monocytes of BALB/c mice were divided into four groups. (1) the cells were cultured in the medium containing LPS 100μg/ml 6 hours after incubation with the Radix-Isatidis extract from 30 min at a final concentration as 1mg/ml; (2) the cells were cultured with Staphylococcus aureus after incubation with the Radix-Isatidis extract 30 min at a final concentration of 1mg/ml; (3) the cells was cultured in the medium containing LPS 100μg/ml for 6 hours after incubation 30 min with DMEM; (4) the cells were cultured for 6 hours in the medium without LPS and any other drugs. TNF, IL-6, NO values were detected in the supernatant, p38 MAPK activity was measured in cells. Then after culture for 6 hours with Radix-Isatidis concentrations diluted to 1∶8, plus LPS 100μg/ml, the p38 MAPK activity was detected. Results There was no obvious increases about p38 MAPK activity and TNF, IL-6, NO levels in the monocytes adding Radix-Isatidis extract, but they increased significantly in the cell cultures with LPS only, the difference was very significant ( P <0.01). When cultured with Staphylococcal aureus , these parameters were increased, too. After Radix-Isatidis concentration diluted, p38 MAPK activities increased gradually. Conclusion Radix-Isatidis inhibit p38 MAPK activity triggered by LPS in dose dependent fashion.

关 键 词：板蓝根 脂多糖 诱导 P38蛋白激酶 活性 中药 

分 类 号：R543[医药卫生—心血管疾病]